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Identification of Novel Allelic Variants of Integrin Beta 2 (ITGB2) Gene and Screening for Bubaline Leukocyte Adhesion Deficiency Syndrome in Indian Water Buffaloes (Bubalus bubalis)
- Source :
- Animal Biotechnology. 20:156-160
- Publication Year :
- 2009
- Publisher :
- Informa UK Limited, 2009.
-
Abstract
- A fragment of 570 bp corresponding to exon 5 and 6 of integrin beta 2 (ITGB2) gene was amplified for screening D128G mutation in one hundred and fifty two buffaloes (Bubalus bubalis) which causes bovine leukocyte adhesion deficiency syndrome (BLAD) in cattle, as well as to ascertain polymorphism. TaqI PCR-RFLP revealed no such mutation thus indicating the absence of bubaline leukocyte adhesion deficiency (BuLAD) allele in animals under study. However, the polymorphism studies using MspI restriction enzyme revealed two genotypic patterns viz. AA pattern (bands of 293, 141, 105, and 31 bp) and BB pattern (bands of 293, 105, 77, 64, and 31 bp). The sequences of A and B alleles were submitted to the GenBank (EU853307 and AY821799).
- Subjects :
- Buffaloes
TaqI
DNA Mutational Analysis
Leukocyte-Adhesion Deficiency Syndrome
Molecular Sequence Data
India
Bioengineering
Biology
chemistry.chemical_compound
Exon
Species Specificity
Genotype
medicine
Animals
Genetic Testing
Leukocyte disorder
Allele
Alleles
DNA Primers
Leukocyte adhesion deficiency
Genetics
Base Sequence
medicine.disease
biology.organism_classification
Molecular biology
Restriction enzyme
chemistry
CD18 Antigens
Cattle
Animal Science and Zoology
Bubalus
Polymorphism, Restriction Fragment Length
Biotechnology
Subjects
Details
- ISSN :
- 15322378 and 10495398
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Animal Biotechnology
- Accession number :
- edsair.doi.dedup.....45d4f7170a116b84d820160cd7756705