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Evaluation of a Parchment Document, the 13th Century Incorporation Charter for the City of Krakow, Poland, for Microbial Hazards
- Source :
- Applied and Environmental Microbiology. 82:2620-2631
- Publication Year :
- 2016
- Publisher :
- American Society for Microbiology, 2016.
-
Abstract
- The literature of environmental microbiology broadly discusses issues associated with microbial hazards in archives, but these publications are mainly devoted to paper documents. There are few articles on historical parchment documents, which used to be very important for the development of literature and the art of writing. These studies present a broad spectrum of methods for the assessment of biodeterioration hazards of the parchment document in question. They are based on both conventional microbiological methods and advanced techniques of molecular biology. Here, a qualitative analysis was conducted, based on genetic identification of bacteria and fungi present on the document as well as denaturing gradient gel electrophoresis profiling and examining the destructive potential of isolated microbes. Moreover, the study involved a quantitative and qualitative microbiological assessment of the indoor air in the room where the parchment was kept. The microbes with the highest destructive potential that were isolated from the investigated item were Bacillus cereus and Acinetobacter lwoffii bacteria and Penicillium chrysogenum , Chaetomium globosum , and Trichoderma longibrachiatum fungi. The presence of the B. cereus strain was particularly interesting since, under appropriate conditions, it leads to complete parchment degradation within several days.
- Subjects :
- Paper
0301 basic medicine
Indoor air
Parchment
Biology
Applied Microbiology and Biotechnology
03 medical and health sciences
Broad spectrum
Qualitative analysis
Microbial ecology
RNA, Ribosomal, 16S
Environmental Microbiology
Skin
Manuscripts as Topic
Bacteria
Ecology
Chaetomium globosum
Archives
Denaturing Gradient Gel Electrophoresis
business.industry
Fungi
Charter
History, Medieval
Biotechnology
030104 developmental biology
Air Pollution, Indoor
Luminescent Measurements
Poland
business
Environmental Monitoring
Food Science
Subjects
Details
- ISSN :
- 10985336 and 00992240
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....45d429cb5bc6c07323bf5174408e7fe5