Back to Search Start Over

In vitro and in vivo assessment of a 3D printable gelatin methacrylate hydrogel for bone regeneration applications

In vitro and in vivo assessment of a 3D printable gelatin methacrylate hydrogel for bone regeneration applications

Authors :
Nehar Celikkin
Simone Mastrogiacomo
Weiqiang Dou
Arend Heerschap
Egbert Oosterwijk
X. Frank Walboomers
Wojciech Święszkowski
Source :
Journal of Biomedical Materials Research Part B-Applied Biomaterials, 110, 2133-2145, Journal of Biomedical Materials Research Part B-Applied Biomaterials, 110, 9, pp. 2133-2145
Publication Year :
2022

Abstract

Contains fulltext : 282312.pdf (Publisher’s version ) (Closed access) Bone tissue engineering (BTE) has made significant progress in developing and assessing different types of bio-substitutes. However, scaffolds production through standardized methods, as required for good manufacturing process (GMP), and post-transplant in vivo monitoring still limit their translation into the clinic. 3D printed 5% GelMA scaffolds have been prepared through an optimized and reproducible process in this work. Mesenchymal stem cells (MSC) were encapsulated in the 3D printable GelMA ink, and their biological properties were assessed in vitro to evaluate their potential for cell delivery application. Moreover, in vivo implantation of the pristine 3D printed GelMA has been performed in a rat condyle defect model. Whereas optimal tissue integration was observed via histology, no signs of fibrotic encapsulation or inhibited bone formation were attained. A multimodal imaging workflow based on computed tomography (CT) and magnetic resonance imaging (MRI) allowed the simultaneous monitoring of both new bone formation and scaffold degradation. These outcomes point out the direction to undertake in developing 3D printed-based hydrogels for BTE that can allow a faster transition into clinical use.

Details

ISSN :
15524981 and 15524973
Volume :
110
Issue :
9
Database :
OpenAIRE
Journal :
Journal of biomedical materials research. Part B, Applied biomaterialsREFERENCES
Accession number :
edsair.doi.dedup.....45cd042f7499d09b0228b5ba7bb2641d