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In vitro and in vivo assessment of a 3D printable gelatin methacrylate hydrogel for bone regeneration applications
In vitro and in vivo assessment of a 3D printable gelatin methacrylate hydrogel for bone regeneration applications
- Source :
- Journal of Biomedical Materials Research Part B-Applied Biomaterials, 110, 2133-2145, Journal of Biomedical Materials Research Part B-Applied Biomaterials, 110, 9, pp. 2133-2145
- Publication Year :
- 2022
-
Abstract
- Contains fulltext : 282312.pdf (Publisher’s version ) (Closed access) Bone tissue engineering (BTE) has made significant progress in developing and assessing different types of bio-substitutes. However, scaffolds production through standardized methods, as required for good manufacturing process (GMP), and post-transplant in vivo monitoring still limit their translation into the clinic. 3D printed 5% GelMA scaffolds have been prepared through an optimized and reproducible process in this work. Mesenchymal stem cells (MSC) were encapsulated in the 3D printable GelMA ink, and their biological properties were assessed in vitro to evaluate their potential for cell delivery application. Moreover, in vivo implantation of the pristine 3D printed GelMA has been performed in a rat condyle defect model. Whereas optimal tissue integration was observed via histology, no signs of fibrotic encapsulation or inhibited bone formation were attained. A multimodal imaging workflow based on computed tomography (CT) and magnetic resonance imaging (MRI) allowed the simultaneous monitoring of both new bone formation and scaffold degradation. These outcomes point out the direction to undertake in developing 3D printed-based hydrogels for BTE that can allow a faster transition into clinical use.
- Subjects :
- Bone Regeneration
Tissue Engineering
Tissue Scaffolds
Biomedical Engineering
Bioprinting
Hydrogels
Rats
Biomaterials
Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]
Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15]
Printing, Three-Dimensional
Animals
Gelatin
Methacrylates
Subjects
Details
- ISSN :
- 15524981 and 15524973
- Volume :
- 110
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of biomedical materials research. Part B, Applied biomaterialsREFERENCES
- Accession number :
- edsair.doi.dedup.....45cd042f7499d09b0228b5ba7bb2641d