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Direct electrochemistry of porcine purple acid phosphatase (uteroferrin)
- Source :
- Biochemistry. 43(32)
- Publication Year :
- 2004
-
Abstract
- Cyclic voltammetry of the non-heme diiron enzyme porcine purple acid phosphatase (uteroferrin, Uf) has been reported for the first time. Totally reversible one-electron oxidation responses (FeIII-FeII f FeIII-FeIII) are seen both in the absence and in the presence of weak competitive inhibitors phosphate and arsenate, and dissociation constants of these oxoanion complexes formed with uteroferrin in its oxidized state (Ufo) have been determined. The effect of pH on the redox potentials has been investigated in the range 3 < pH < 6.5, enabling acid dissociation constants for Ufo and its phosphate and arsenate complexes to be calculated.
- Subjects :
- Swine
Iron
Inorganic chemistry
Acid Phosphatase
Biochemistry
Redox
Binding, Competitive
Acid dissociation constant
Phosphates
Electron Transport
chemistry.chemical_compound
Electrochemistry
Animals
Enzyme Inhibitors
Glycoproteins
biology
Arsenate
Active site
Purple acid phosphatases
Hydrogen-Ion Concentration
Phosphate
Dissociation constant
Chemistry
chemistry
biology.protein
Arsenates
Cyclic voltammetry
Oxidation-Reduction
Nuclear chemistry
Subjects
Details
- ISSN :
- 00062960
- Volume :
- 43
- Issue :
- 32
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....45be5f6f5bcfa59e4194ebde41ada971