Back to Search
Start Over
Effect of 3,4-di(OH)-cinnamate synthetic derivative on plasma and hepatic cholesterol level and antioxidant enzyme activities in high cholesterol-fed rats
- Source :
- Journal of Biochemical and Molecular Toxicology. 18:279-287
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- The effect of 3,4-di(OH)-phenylpropionic acid (L-phenylalanine methyl ester) amide (SL-1063), a synthetic derivative of 3,4-di(OH)-cinnamate, on the cholesterol metabolism and antioxidant enzyme system was examined in rats. Diets that included either SL-1063 (0.046%, w/w) or lovastatin (0.02%, w/w) as a supplement, plus 1 g cholesterol/100 g diet were fed to rats ad libitum for 5 weeks. The total plasma cholesterol and triglyceride levels were significantly lowered by the SL-1063 supplement compared to the control group. Meanwhile, the levels of plasma HDL-cholesterol and ratio of HDL-cholesterol/total cholesterol (%) were significantly higher in the SL-1063 group than in the control group. However, the lovastatin supplement did not affect the plasma lipid level. The hepatic cholesterol level and 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase activity were significantly lowered in the lovastatin group compared to the SL-1063 group; however, the hepatic triglyceride level did not differ among the groups. The activity of hepatic acyl CoA: cholesterol acyltransferase (ACAT), the enzyme that catalyzes hepatic cholesterol esterification, was significantly lower in the lovastatin and SL-1063 groups than in the control group. Furthermore, the SL-1063 supplement elevated the excretion of fecal sterols. As regards the hepatic antioxidant enzyme system, the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione reductase (GR) activities were all significantly higher in the SL-1063 group compared to the control group, whereas only the GR activity was significantly increased by the lovastatin supplement. No marked difference in the GSH levels and glucose-6-phosphate dehydrogenase (G6PD) activities was observed among the groups. The levels of plasma and hepatic thiobarbituric acid reactive substances (TBARS) were lowered by the SL-1063 supplement compared to the control group. Accordingly, the current results suggest that SL-1063, a synthetic derivative of 3,4-di(OH)-cinnamate, is effective in lowering the plasma lipids and improving the antioxidant enzyme system.
- Subjects :
- Male
medicine.medical_specialty
Coumaric Acids
Phenylalanine
Health, Toxicology and Mutagenesis
Glutathione reductase
Sterol O-acyltransferase
Toxicology
Biochemistry
High cholesterol
Cholesterol, Dietary
Rats, Sprague-Dawley
chemistry.chemical_compound
Caffeic Acids
Internal medicine
medicine
TBARS
Animals
Lovastatin
Molecular Biology
chemistry.chemical_classification
Glutathione Peroxidase
Triglyceride
Superoxide Dismutase
Cholesterol
Glutathione peroxidase
General Medicine
medicine.disease
Rats
Glutathione Reductase
Endocrinology
Liver
chemistry
Microsomes, Liver
Molecular Medicine
Hydroxymethylglutaryl CoA Reductases
lipids (amino acids, peptides, and proteins)
Sterol O-Acyltransferase
medicine.drug
Subjects
Details
- ISSN :
- 10990461 and 10956670
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Biochemical and Molecular Toxicology
- Accession number :
- edsair.doi.dedup.....45be4db3fff2d0cec529c416704d40af