Back to Search
Start Over
Detection of Hb Anti-Lepore Hong Kong (NG_000007.3: g.63154_70565dup) in Chinese Individuals
- Source :
- Hemoglobin. 38:146-148
- Publication Year :
- 2014
- Publisher :
- Informa UK Limited, 2014.
-
Abstract
- We have identified four Chinese individuals from three unrelated families with raised Hb A2 levels. The anti-Lepore hybrid hemoglobin (Hb) variant was amplified using a pair of primers, 5' to the β-globin gene Cap site and 3' to the δ-globin gene polyadenylation site (polyA) region, respectively. Direct sequencing of the βδ fusion products confirmed the anti-Lepore Hong Kong (NG_000007.3: g.63154_70565dup) variant. We found that this anti-Lepore variant is positioned in zone 3 on the capillary electrophoresis system. It may help in differential diagnosis of Hb variants and providing better information in clinical counseling.
- Subjects :
- Adult
Male
China
Hemoglobins, Abnormal
Recombinant Fusion Proteins
DNA Mutational Analysis
Clinical Biochemistry
beta-Globins
Biology
medicine.disease_cause
Young Adult
Polyadenylation site
Hb anti-Lepore Hong Kong
medicine
Humans
Hemoglobin A2
Gene
Genetics (clinical)
Family Health
Genetics
Family health
delta-Globins
Mutation
Direct sequencing
Biochemistry (medical)
Electrophoresis, Capillary
Hematology
Middle Aged
Molecular biology
Female
Hemoglobin
Subjects
Details
- ISSN :
- 1532432X and 03630269
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Hemoglobin
- Accession number :
- edsair.doi.dedup.....45b5034915459899b5abc2c68076d4a7