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Chimeric receptors as a tool for luminescent measurement of biological activities of steroid hormones
- Source :
- Journal of bioluminescence and chemiluminescence. 9(3)
- Publication Year :
- 1994
-
Abstract
- Some applications of chimeric cellular models are presented to study the biological activities of steroid hormones. We have used several chimeric constructs encoding the DNA binding domain of Gal4 yeast protein fused to the hormone binding domain of various steroid receptors (MR, PR, GR and ER). Interactions of these chimeric receptors with a 17-mer DNA sequence, specific for Gal-4, control expression of the firefly luciferase as a reporter gene. Stable transfected cell lines expressing the firefly luciferase under the control of different steroids were established and an efficient and easy sub-cloning was allowed with the help of an imaging system using a single-photon-counting camera. In the cell lines obtained, the bioluminescent response can be easily measured and thus used to measure specific biological activities of steroid agonists or antagonists. We observed that the responses are effector-concentration-dependent and their biological activities will be compared to those of native receptors.
- Subjects :
- Receptors, Steroid
Saccharomyces cerevisiae Proteins
medicine.medical_treatment
Recombinant Fusion Proteins
Biophysics
Biology
Transfection
Steroid
Cell Line
Fungal Proteins
medicine
Animals
Humans
Luciferase
Receptor
Luciferases
Reporter gene
Binding Sites
DNA-binding domain
DNA
Coleoptera
DNA-Binding Proteins
Biochemistry
Chemistry (miscellaneous)
Luminescent Measurements
Steroids
Hormone
Binding domain
HeLa Cells
Plasmids
Transcription Factors
Subjects
Details
- ISSN :
- 08843996
- Volume :
- 9
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of bioluminescence and chemiluminescence
- Accession number :
- edsair.doi.dedup.....45a7704337bca8a4b37bacd631759813