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Small-Animal 18F-FDG PET for Research on Octopus vulgaris: Applications and Future Directions in Invertebrate Neuroscience and Tissue Regeneration
- Source :
- The Journal of nuclear medicine (1978) 59 (2018): 1302–1307. doi:10.2967/jnumed.117.205393, info:cnr-pdr/source/autori:Letizia Zullo 1, Ambra Buschiazzo 2, Michela Massollo 3, Mattia Riondato 4, Alessia Democrito 4, Cecilia Marini 2,4,5, Fabio Benfenati 1,6 and Gianmario Sambuceti 2,4,5/titolo:Small-Animal F-18-FDG PET for Research on Octopus vulgaris: Applications and Future Directions in Invertebrate Neuroscience and Tissue Regeneration/doi:10.2967%2Fjnumed.117.205393/rivista:The Journal of nuclear medicine (1978)/anno:2018/pagina_da:1302/pagina_a:1307/intervallo_pagine:1302–1307/volume:59
- Publication Year :
- 2018
- Publisher :
- Society of Nuclear Medicine, 2018.
-
Abstract
- This study aimed to develop a method of administering F-18-FDG to the common octopus in order to perform a PET biodistribution assay characterizing glucose metabolism in organs and regenerating tissues. Methods: Seven animals (two of which had a regenerating arm) were anesthetized with 3.7% MgCl2 in artificial seawater and then injected with 18-30 MBq of isosmotic F-18-FDG through either the left branchial heart or the anterior vena cava. After an uptake time of about 50 min, the animals were sacrificed and placed on the bed of a small-animal PET scanner, and 10-min static acquisitions were obtained at 3-4 bed positions to visualize the entire body. To confirm image interpretation, internal organs of interest were collected and counted with a.-counter. Results: Administration through the anterior vena cava resulted in a good full-body distribution of F-18-FDG as seen on the PET images. Uptake was high in the mantle mass and relatively lower in the arms. In particular, the brain, optic lobes, and arms were clearly identified and were measured for their uptake (SUVmax: 6.57 +/- 1.86, 7.59 +/- 1.66, and 1.12 +/- 0.06, respectively). Interestingly, F-18-FDG uptake was up to 3-fold higher in the highly proliferating areas of regenerating arms. Conclusion: This study represents a stepping-stone to the use of non-invasive functional techniques for addressing questions about invertebrate neuroscience and regenerative medicine.
- Subjects :
- 0301 basic medicine
Biodistribution
biology
Regeneration (biology)
Common octopus
biology.organism_classification
Regenerative medicine
18f fdg pet
F-18-FDG
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Branchial heart
regeneration
Small animal
micro-PET
Radiology, Nuclear Medicine and imaging
octopus
Mantle (mollusc)
Neuroscience
030217 neurology & neurosurgery
functional imaging
Subjects
Details
- ISSN :
- 2159662X and 01615505
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Journal of Nuclear Medicine
- Accession number :
- edsair.doi.dedup.....45a68d608499e613d83c317a032cd1c1