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Uptake and turnover of dopamine in rat mast cells studied by cytofluorometry and high performance liquid chromatography
- Source :
- The Histochemical journal. 14(3)
- Publication Year :
- 1982
-
Abstract
- Uptake and turnover of dopamine (DA) in rat peritoneal mast cells were studied by a cytofluorometric technique. The main advantage of the method is that it permits the study of the distribution of amine content within populations of cells. Catecholamines and indolamines can be differentiated, but subtler structural differences in this group of compounds cannot be distinguished. We, therefore, combined the cytofluorometric measurements with a liquid chromatographic method based on reversed-phase chromatography followed by amperometric detection in a thin layer flow cell. Intraperitoneally injected L-DOPA was rapidly decarboxylated to DA, which was accumulated in mast cell granules. The elimination of DA from the mast cells was much faster than previously published 5-hydroxytryptamine and histamine elimination rates. No evidence of intracellular conversion of DA before its elimination was found and simultaneous heparin quantitations gave no evidence of an elimination pathway due to exocytosis of granules. Electron microscopy disclosed no structural changes that could be related to exocytosis during the elimination phase of DA. The rapid elimination together with absence of inhibition of DA-uptake after storage of exogenous 5-hydroxytryptamine suggest that the mechanism of DA storage differs from the mechanism of storage of endogenous mast cell amines.
- Subjects :
- Male
Serotonin
Dopamine
Endogeny
High-performance liquid chromatography
Exocytosis
Levodopa
chemistry.chemical_compound
medicine
Animals
Fluorometry
Mast Cells
Chromatography, High Pressure Liquid
Rats, Inbred Strains
Cell Biology
Mast cell
Rats
Kinetics
medicine.anatomical_structure
Biochemistry
Indolamines
chemistry
Anatomy
Intracellular
Histamine
medicine.drug
Subjects
Details
- ISSN :
- 00182214
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The Histochemical journal
- Accession number :
- edsair.doi.dedup.....45a347181a17df309fb9be91dd22fc46