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Protective Effect of Heat Shock Protein 70 Against Oxidative Stresses in Human Corneal Fibroblasts
- Source :
- Journal of Korean Medical Science
- Publication Year :
- 2004
- Publisher :
- Korean Academy of Medical Sciences, 2004.
-
Abstract
- We evaluated DNA protection effect of heat shock protein (HSP) against cytotoxic effects of exogenous nitric oxide (NO) and reactive oxygen intermediate (ROI). Cultured human corneal fibroblasts were divided into 4 groups. Control (Group I) was not exposed to a sub-lethal heat treatment. Other 3 groups were exposed to 43 degrees C for 1 hr, then incubated at 37 degrees C during different duration (1, 6, 24 hr, Group II, III, IV, respectively). Expression pattern of HSP 70 was analyzed by Western blot. Cell viability was measured by MTT assay and the relationship between HSP 70 expression and DNA damage was examined by terminal deoxyribonucleotidyl transferase mediated dUTP-digoxigenin nick and labeling (TUNEL) stain and single cell gel electrophoresis. Expression pattern of HSP 70 was dependent on recovery times. Cell viability following heat treatment was significantly increased and the TUNEL positive cell number was decreased at 6 hr. In single cell gel electrophoresis, tail moments were increased in a dose-dependent manner by SNAP and X/XO. Following heat treatment, tail moments showed decreased significantly at 6 hr. These results suggest that induction of HSP 70 by sub-lethal heat treatment is closely related with cytoprotective effects against oxidative stresses in human corneal fibroblasts.
- Subjects :
- Xanthine Oxidase
Hot Temperature
Cell Survival
DNA damage
In-Situ Nick-End Labeling
S-Nitroso-N-Acetylpenicillamine
Biology
Nitric Oxide
medicine.disease_cause
Xanthine
Heat Shock Proteins 70
Cornea
Western blot
Heat shock protein
In Situ Nick-End Labeling
medicine
Humans
HSP70 Heat-Shock Proteins
Nitric Oxide Donors
MTT assay
Viability assay
Cells, Cultured
TUNEL assay
Dose-Response Relationship, Drug
medicine.diagnostic_test
General Medicine
Fibroblasts
Molecular biology
Hsp70
Oxidative Stress
Original Article
Reactive Oxygen Species
Oxidative stress
DNA Damage
Subjects
Details
- ISSN :
- 10118934
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Journal of Korean Medical Science
- Accession number :
- edsair.doi.dedup.....457bf6358ede4dcb84d96f5af80cb617