Back to Search
Start Over
Study on the Immunoreactivity of Triticum monococcum (Einkorn) Wheat in Patients with Wheat-Dependent Exercise-Induced Anaphylaxis for the Production of Hypoallergenic Foods
- Source :
- Journal of agricultural and food chemistry. 63(37)
- Publication Year :
- 2015
-
Abstract
- Wheat [Triticum aestivum (T.a.)] ingestion can cause a specific allergic reaction, which is called wheat-dependent exercise-induced anaphylaxis (WDEIA). The major allergen involved is ω-5 gliadin, a gluten protein coded by genes located on the B genome. Our aim was to study the immunoreactivity of proteins in Triticum monococcum (einkorn, T.m.), a diploid ancestral wheat lacking B chromosomes, for possible use in the production of hypoallergenic foods. A total of 14 patients with a clear history of WDEIA and specific immunoglobulin E (IgE) to ω-5 gliadin were enrolled. Skin prick test (SPT) with a commercial wheat extract and an in-house T.a. gluten diagnostic solution tested positive for 43 and 100% of the cases, respectively. No reactivity in patients tested with solutions prepared from four T.m. accessions was observed. The immunoblotting of T.m. gluten proteins performed with the sera of patients showed different IgE-binding profiles with respect to T.a., confirming the absence of ω-5 gliadin. A general lower immunoreactivity of T.m. gluten proteins with scarce cross-reactivity to ω-5 gliadin epitopes was assessed by an enzyme-linked immunosorbent assay (ELISA). Given the absence of reactivity by SPT and the limited cross-reactivity with ω-5 gliadin, T.m. might represent a potential candidate in the production of hypoallergenic bakery products for patients sensitized to ω-5 gliadin. Further analyses need to be carried out regarding its safety.
- Subjects :
- Triticum monococcum
Male
Flour
Wheat Hypersensitivity
Immunoglobulin E
medicine.disease_cause
Epitope
Gliadin
Epitopes
Allergen
wheat
Triticum
chemistry.chemical_classification
biology
food and beverages
Middle Aged
Female
Ploidy
General Agricultural and Biological Sciences
Anaphylaxis
Adult
Glutens
Enzyme-Linked Immunosorbent Assay
Chromosomes, Plant
wheat-dependent exercise-induced anaphylaxis (WDEIA)
medicine
Humans
Exercise
Skin Tests
business.industry
Plant Extracts
nutritional and metabolic diseases
Hypoallergenic
General Chemistry
Allergens
Antigens, Plant
medicine.disease
allergy
Gluten
digestive system diseases
Biotechnology
ω gliadin
chemistry
Immunology
biology.protein
business
Subjects
Details
- ISSN :
- 15205118
- Volume :
- 63
- Issue :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of agricultural and food chemistry
- Accession number :
- edsair.doi.dedup.....4572ee684f3ca8e4d86e34c07e6c0e77