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Defenses of Susceptible and Resistant Chinook Salmon (Onchorhynchus tshawytscha) Against the Myxozoan Parasite Ceratomyxa shasta
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2014
-
Abstract
- We investigated intra-specific variation in the response of salmon to infection with the myxozoan Ceratomyxa shasta by comparing the progress of parasite infection and measures of host immune response in susceptible and resistant Chinook salmon Oncorhynchus tshawytscha at days 12, 25 and 90 post exposure. There were no differences in invasion of the gills indicating that resistance does not occur at the site of entry. In the intestine on day 12, infection intensity and Ig+ cell numbers were higher in susceptible than resistant fish, but histological examination at that timepoint showed more severe inflammation in resistant fish. This suggests a role for the immune response in resistant fish that eliminates some parasites prior to or soon after reaching the intestine. Susceptible fish had a higher IFNγ, IL-6 and IL-10 response at day 12, but all died of fatal enteronecrosis by day 25. The greatest fold change in IFNγ expression was detected at day 25 in resistant Chinook. In addition, the number of Ig+ cells in resistant Chinook also increased by day 25. By day 90, resistant Chinook had resolved the inflammation, cytokine expression had decreased and Ig+ cell numbers were similar to uninfected controls. Thus, it appears that the susceptible strain was incapable of containing or eliminating C.shasta but resistant fish: 1) reduced infection intensity during early intestinal infection, 2) elicited an effective inflammatory response in the intestine that eliminated C.shasta, 3) resolved the inflammation and recovered from infection. © 2014 Elsevier Ltd.
- Subjects :
- Gill
Gills
Time Factors
medicine.medical_treatment
Parasitic Diseases, Animal
Resistance
Inflammation
Aquatic Science
Real-Time Polymerase Chain Reaction
Article
Microbiology
Fish Diseases
Immune system
Species Specificity
Ceratomyxa shasta
Salmon
medicine
Environmental Chemistry
Parasite hosting
Animals
Immune response
Myxozoa
Cytokine
DNA Primers
Disease Resistance
biology
General Medicine
biology.organism_classification
Immunohistochemistry
Intestines
Immunology
Oncorhynchus
Cytokines
medicine.symptom
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....4564a9749e0ecb5216ea586fbfc81aef