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Detection of quebec polyomavirus dna in samples from different patient groups
- Source :
- Microorganisms, Microorganisms, Vol 9, Iss 1082, p 1082 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Polyomaviruses infect many species, including humans. So far, 15 polyomaviruses have been described in humans, but it remains to be established whether all of these are genuine human polyomaviruses. The most recent polyomavirus to be detected in a person is Quebec polyomavirus (QPyV), which was identified in a metagenomic analysis of a stool sample from an 85-year-old hospitalized man. We used PCR to investigate the presence of QPyV DNA in urine samples from systemic lupus erythematosus (SLE) patients (67 patients; 135 samples), multiple sclerosis patients (n = 35), HIV-positive patients (n = 66) and pregnant women (n = 65). Moreover, cerebrospinal fluid from patients with suspected neurological diseases (n = 63), nasopharyngeal aspirates from patients (n = 80) with respiratory symptoms and plasma samples from HIV-positive patients (n = 65) were examined. QPyV DNA was found in urine from 11 (16.4%), 10 (15.4%) and 5 (14.3%) SLE patients, pregnant women, and multiple sclerosis patients, respectively. No QPyV DNA could be detected in the other samples. Alignment with the only available QPyV sequence in the GenBank revealed amino acid substitutions in the HI-loop of capsid protein VP1 in 6/28 of the isolates. Our results show that QPyV viruria can occur, but whether it may cause clinical symptoms in the patients remains to be determined.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
Stool sample
QH301-705.5
030106 microbiology
Urine
multiple sclerosis
Microbiology
Gastroenterology
cerebrospinal fluid
03 medical and health sciences
chemistry.chemical_compound
Cerebrospinal fluid
systemic lupus erythematosus
Virology
Internal medicine
HI-loop
medicine
Biology (General)
Plasma samples
business.industry
Multiple sclerosis
Communication
nasopharyngeal aspirates
HIV
VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710
VP1
medicine.disease
urine
VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710
hi-loop
hiv
vp1
pregnant women
030104 developmental biology
chemistry
Capsid
GenBank
business
DNA
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Microorganisms, Microorganisms, Vol 9, Iss 1082, p 1082 (2021)
- Accession number :
- edsair.doi.dedup.....4557ae611b4554987432b324ffc96f0b