Back to Search
Start Over
Biochemical and molecular biomarkers in integument biopsies of free-ranging coastal bottlenose dolphins from southern Brazil
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2019
-
Abstract
- Adverse effects of exposure to persistent organic pollutants (POPs) threaten the maintenance of odontocete populations. In southern Brazil, coastal bottlenose dolphins from the Laguna Estuarine System (LES) and Patos Lagoon Estuary (PLE) were sampled using remote biopsies during the winter and summer months. Levels of bioaccumulated POPs were measured in the blubber. The activities of glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also quantified, as were the mRNA transcript levels of aryl hydrocarbon receptor (AhR), AhR nuclear translocator (ARNT), cytochrome P450 1A1-like (CYP1A1), metallothionein 2A (MT2A), GST-π, GPx-4, GR, interleukin 1 alpha (IL-1α), and major histocompatibility complex II (MHCII) in the skin. In general, levels of POPs were similar among sites, sexes, ages and seasons. For most animals, total polychlorinated biphenyl (ΣPCBs) levels were above the threshold level have physiological effects and pose risks to cetaceans. The best-fitting generalized linear models (GLMs) found significant associations between GR, IL-1α and GPx-4 transcript levels, SOD and GST activities, and total polybrominated diphenyl ether (ΣPBDEs) and pesticide levels. GLMs and Kruskal-Wallis analyses also indicated that there were higher transcript levels for most genes and lower GST activity in the winter. These results reinforce the need to consider the influence of environmental traits on biomarker values in wildlife assessments.
- Subjects :
- Environmental Engineering
Aryl hydrocarbon receptor nuclear translocator
Biopsy
Health, Toxicology and Mutagenesis
0208 environmental biotechnology
Glutathione reductase
Zoology
02 engineering and technology
010501 environmental sciences
Biology
01 natural sciences
Gene Expression Regulation, Enzymologic
chemistry.chemical_compound
Blubber
POLUIÇÃO AMBIENTAL
Animals
Environmental Chemistry
RNA, Messenger
0105 earth and related environmental sciences
chemistry.chemical_classification
Glutathione peroxidase
Public Health, Environmental and Occupational Health
Polychlorinated biphenyl
General Medicine
General Chemistry
Glutathione
Pesticide
Aryl hydrocarbon receptor
Pollution
020801 environmental engineering
Bottle-Nosed Dolphin
Adipose Tissue
chemistry
biology.protein
Biomarkers
Brazil
Water Pollutants, Chemical
Environmental Monitoring
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Accession number :
- edsair.doi.dedup.....455725482ee97cc921bdb6952fbd25fd