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The effect of bovine herpesvirus type 1 glycoproteins gI and gIII on herpesvirus infections
- Source :
- The Journal of general virology. 70
- Publication Year :
- 1989
-
Abstract
- Summary We expressed the bovine herpesvirus type 1 (BHV-1) glycoproteins, gI and gIII, in bovine cells using a bovine papillomavirus vector. The proteins expressed by these cells had the same M r as the native BHV-1 proteins and monoclonal antibodies detected no differences in their antigenic structure. Cells expressing gI were infected with either BHV-1 or herpes simplex virus type 1 (HSV-1). The number of plaques in gI-expressing cells was similar to that seen with normal fibroblasts infected with BHV-1 or HSV-1. However, BHV-1 or HSV-1 plaques produced in gI-expressing cells were smaller and darker than those seen in normal fibroblasts indicating an interference with cell-to-cell transmission or cellular lysis. Virus growth curves and [35S]methionine labelling of BHV-1-infected gI-expressing cells showed no difference in virus production, virus protein synthesis or cellular protein shutdown when compared to BHV-1-infected normal cells. This led us to conclude that the gI protein may interfere with a cellular protein(s) responsible for the cytopathic effects of BHV-1 infection. Cells expressing gIII were fully susceptible to BHV-1 infection.
- Subjects :
- animal diseases
viruses
Genetic Vectors
Viral Plaque Assay
medicine.disease_cause
Herpesviridae
Virus
Viral Proteins
Antigen
Virology
medicine
Animals
Simplexvirus
Cells, Cultured
Bovine papillomavirus
Bovine papillomavirus 1
Herpesvirus 1, Bovine
chemistry.chemical_classification
biology
virus diseases
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Molecular biology
Bovine herpesvirus 1
Recombinant Proteins
Herpes simplex virus
chemistry
Gene Expression Regulation
Cell culture
Cattle
Glycoprotein
Plasmids
Subjects
Details
- ISSN :
- 00221317
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- The Journal of general virology
- Accession number :
- edsair.doi.dedup.....455311e73d9449548e78e1d906a566f8