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Use of [75Se]selenomethionine in immunoglobulin biosynthetic studies
- Source :
- Journal of immunological methods. 21(1-2)
- Publication Year :
- 1978
-
Abstract
- The gamma-emitting amino acid analog, [75Se]selenomethionine, has been used as a biosynthetic label for immunoglobulins secreted by plasmacytomas in tissue culture. The secreted products are structurally intact with respect to their antibody combining sites and their class and allotype antigenic specificities. A component of [75Se]selenomethionine preparations was found to bind to fetal calf serum proteins, in a manner releasable by mercaptoethanol, but not by sodium dodecyl sulfate (SDS) and urea. Methods for circumventing the problems caused by this binding are described.
- Subjects :
- Phosphorylcholine
Immunology
Radioimmunoassay
Immunoglobulins
Biology
chemistry.chemical_compound
Tissue culture
Mice
Selenium
Antigen
Immunology and Allergy
Animals
Sodium dodecyl sulfate
Selenomethionine
chemistry.chemical_classification
Mice, Inbred BALB C
Mice, Inbred NZB
Metabolism
Molecular biology
Blood proteins
Allotype
Amino acid
Culture Media
chemistry
Biochemistry
biology.protein
Electrophoresis, Polyacrylamide Gel
Antibody
Immunosorbents
Multiple Myeloma
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 21
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Journal of immunological methods
- Accession number :
- edsair.doi.dedup.....454c7141d044f7a1cf08bb39a9c8d604