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Noninvasive assessment of the functional neovasculature in 9L-glioma growing in rat brain by dynamic 1H magnetic resonance imaging of gadolinium uptake
- Source :
- Journal of Cerebral Blood Flow and Metabolism, 20, 5, pp. 861-870, Journal of Cerebral Blood Flow and Metabolism, 20, 861-870
- Publication Year :
- 2000
-
Abstract
- Pathophysiologic parameters of the functional neovasculature and the blood-brain barrier of 9L-glioma in rat brain were measured noninvasively by dynamic 1H magnetic resonance imaging studies of gadolinium (Gd)-DTPA uptake. Changes of apparent [Gd-DTPA] uptake in time (CT[t]) were analyzed in a slice through the center of 10 9L-gliomas using fast T1 measurements. The distribution of the contrast agent was spatially correlated with the distribution of perfused microvessels as determined by immunohistochemical analysis. This method permits a distinction between perfused and nonperfused microvessels with a disrupted blood-brain barrier. In transverse slices of the whole tumor, a spatial correlation was observed between CT maps and the two-dimensional distribution of perfused microvessels. In the next step, Gd-DTPA uptake rates were spatially related to the perfused microvessel density (Np) or vascular surface area (Sp). In tumor voxels with perfused microvessels, a linear correlation was found between Gd-DTPA uptake rate constants (k values) and Np or Sp. No correlation was observed between k values and the total microvessel density. These are the first data that show a relation between Gd-DTPA uptake rates and parameters of the functional neovasculature in 9L-glioma growing in rat brain. Now that Gd-DTPA uptake studies can be related to parameters of the functional neovasculature, they may be used more efficiently as a prognostic tool before or during therapy.
- Subjects :
- Gadolinium DTPA
Pathology
medicine.medical_specialty
Gadolinium
Contrast Media
chemistry.chemical_element
computer.software_genre
030218 nuclear medicine & medical imaging
Neovascularization
03 medical and health sciences
0302 clinical medicine
Nuclear magnetic resonance
Voxel
Glioma
Biomedische Magnetische Resonantie
medicine
Fluorescence microscope
Animals
Distribution (pharmacology)
Experimental radiotherapy and neuro-oncology
Neovascularization, Pathologic
medicine.diagnostic_test
Brain Neoplasms
Chemistry
Magnetic resonance imaging
medicine.disease
Experimentele radiotherapie en neuro-oncologie
Immunohistochemistry
Magnetic Resonance Imaging
Rats, Inbred F344
Rats
Biomedical Magnetic Resonance
Neurology
cardiovascular system
Neurology (clinical)
Protons
medicine.symptom
Cardiology and Cardiovascular Medicine
computer
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 0271678X
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Journal of Cerebral Blood Flow and Metabolism
- Accession number :
- edsair.doi.dedup.....45258e9abb5d318ab3b7244e184f4e61