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The Drosophila PRR GNBP3 assembles effector complexes involved in antifungal defenses independently of its Toll-pathway activation function
- Source :
- European Journal of Immunology, European Journal of Immunology, Wiley-VCH Verlag, 2010, 40 (5), pp.1244-1254. ⟨10.1002/eji.200940164⟩, European Journal of Immunology, 40, 5, pp. 1244-54, European Journal of Immunology, 40, 1244-54, European Journal of Immunology, 2010, 40 (5), pp.1244-1254. ⟨10.1002/eji.200940164⟩
- Publication Year :
- 2010
-
Abstract
- Item does not contain fulltext The Drosophila Toll-signaling pathway controls the systemic antifungal host response. Gram-negative binding protein 3 (GNBP3), a member of the beta-glucan recognition protein family senses fungal infections and activates this pathway. A second detection system perceives the activity of proteolytic fungal virulence factors and redundantly activates Toll. GNBP3(hades) mutant flies succumb more rapidly to Candida albicans and to entomopathogenic fungal infections than WT flies, despite normal triggering of the Toll pathway via the virulence detection system. These observations suggest that GNBP3 triggers antifungal defenses that are not dependent on activation of the Toll pathway. Here, we show that GNBP3 agglutinates fungal cells. Furthermore, it can activate melanization in a Toll-independent manner. Melanization is likely to be an essential defense against some fungal infections given that the entomopathogenic fungus Beauveria bassiana inhibits the activity of the main melanization enzymes, the phenol oxidases. Finally, we show that GNBP3 assembles "attack complexes", which comprise phenoloxidase and the necrotic serpin. We propose that Drosophila GNBP3 targets fungi immediately at the inception of the infection by bringing effector molecules in direct contact with the invading microorganisms. 01 mei 2010
- Subjects :
- [SDV]Life Sciences [q-bio]
MESH: Melanins
Hemolymph
Candida albicans
Immunology and Allergy
Drosophila Proteins
MESH: Animals
MESH: Beauveria
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Fungal protein
Effector
Monophenol Monooxygenase
Toll-Like Receptors
Intracellular Signaling Peptides and Proteins
Spores, Fungal
[SDV] Life Sciences [q-bio]
Drosophila melanogaster
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
[SDV.IMM]Life Sciences [q-bio]/Immunology
Drosophila Protein
MESH: Toll-Like Receptors
MESH: Monophenol Monooxygenase
MESH: Fungi
Agglutination
MESH: Enzyme Activation
[SDV.IMM] Life Sciences [q-bio]/Immunology
MESH: Drosophila Proteins
Recombinant Fusion Proteins
Immunology
Virulence
MESH: Carrier Proteins
Biology
Serpin
Article
Microbiology
MESH: Drosophila melanogaster
Invasive mycoses and compromised host [N4i 2]
MESH: Spores, Fungal
MESH: Intracellular Signaling Peptides and Proteins
MESH: Serpins
MESH: Recombinant Fusion Proteins
Animals
Beauveria
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Serpins
Melanins
Innate immune system
MESH: Candida albicans
MESH: Hemolymph
fungi
Fungi
MESH: Multiprotein Complexes
biology.organism_classification
[SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Enzyme Activation
Metabolic pathway
Multiprotein Complexes
Carrier Proteins
MESH: Agglutination
Subjects
Details
- ISSN :
- 00142980 and 15214141
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....450f12b249eaa385b4e5b9b01ae70d7e