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Global translation variations in host cells upon attack of lytic and sublytic Staphylococcus aureus alpha-haemolysin
- Source :
- Biochemical journal (Online) 472 (2015): 83–95. doi:10.1042/BJ20150284, info:cnr-pdr/source/autori:Clamer, Massimiliano; Tebaldi, Toma; Marchioretto, Marta; Bernabo, Paola; Bertini, Efrem; Guella, Graziano; Dalla Serra, Mauro; Quattrone, Alessandro; Viero, Gabriella/titolo:Global translation variations in host cells upon attack of lytic and sublytic Staphylococcus aureus alpha-haemolysin./doi:10.1042%2FBJ20150284/rivista:Biochemical journal (Online)/anno:2015/pagina_da:83/pagina_a:95/intervallo_pagine:83–95/volume:472
- Publication Year :
- 2015
- Publisher :
- Portland Press, London , Regno Unito, 2015.
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Abstract
- Genome-wide analyses of translation can provide major contributions in our understanding of the complex interplay between virulent factors and host cells. So far, the activation of host translational control mechanisms by bacterial toxins, owing to specific recruitment of mRNAs, RNA-binding proteins (RBPs) and ncRNAs (non-coding RNAs), are far from being understood. In the present study, we characterize for the first time the changes experienced by the translational control system of host cells in response to the well-known Staphylococcus aureus α-haemolysin (AHL) under both sublytic and lytic conditions. By comparing variations occurring in the cellular transcriptome and translatome, we give evidence that global gene expression is primarily rewired at the translational level, with the contribution of the RBP ELAVL1 (HuR) in the sublytic response. These results reveal the importance of translational control during host–pathogen interaction, opening new approaches for AHL-induced diseases.
- Subjects :
- pore-forming toxins
RNA-binding protein
Immunoblotting
translatome
Virulence
Biology
Biochemistry
ELAV-Like Protein 1
Transcriptome
Hemolysin Proteins
Cell Line, Tumor
polysomal profiling
Gene expression
Protein biosynthesis
Humans
Molecular Biology
Oligonucleotide Array Sequence Analysis
bacterial toxins
Genetics
Dose-Response Relationship, Drug
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Genetic Variation
translational control
Translation (biology)
Cell Biology
Gene expression profiling
Lytic cycle
Protein Biosynthesis
Mutation
HuR
transcriptome
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biochemical journal (Online) 472 (2015): 83–95. doi:10.1042/BJ20150284, info:cnr-pdr/source/autori:Clamer, Massimiliano; Tebaldi, Toma; Marchioretto, Marta; Bernabo, Paola; Bertini, Efrem; Guella, Graziano; Dalla Serra, Mauro; Quattrone, Alessandro; Viero, Gabriella/titolo:Global translation variations in host cells upon attack of lytic and sublytic Staphylococcus aureus alpha-haemolysin./doi:10.1042%2FBJ20150284/rivista:Biochemical journal (Online)/anno:2015/pagina_da:83/pagina_a:95/intervallo_pagine:83–95/volume:472
- Accession number :
- edsair.doi.dedup.....45079d7e78dd465b70858fcbf874c93f