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Exercise-dependent increases in protein synthesis are accompanied by chromatin modifications and increased MRTF-SRF signalling
- Source :
- Acta Physiologica (Oxford, England), Solagna, F, Nogara, L, Dyar, K A, Greulich, F, Mir, A A, Türk, C, Bock, T, Geremia, A, Baraldo, M, Sartori, R, Farup, J, Uhlenhaut, H, Vissing, K, Kruger, M & Blaauw, B 2020, ' Exercise-dependent increases in protein synthesis are accompanied by chromatin modifications and increased MRTF-SRF signalling ', Acta Physiologica, vol. 230, no. 1, e13496 . https://doi.org/10.1111/apha.13496, Acta Physiol. 230:e13496 (2020)
- Publication Year :
- 2019
-
Abstract
- AIM: Resistance exercise increases muscle mass over time. However, the early signalling events leading to muscle growth are not yet well-defined. Here, we aim to identify new signalling pathways important for muscle remodelling after exercise METHODS: We performed a phosphoproteomics screen after a single bout of exercise in mice. As an exercise model we used unilateral electrical stimulation in vivo and treadmill running. We analysed muscle biopsies from human subjects to verify if our findings in murine muscle also translate to exercise in humans RESULTS: We identified a new phosphorylation site on Myocardin-Related Transcription Factor B (MRTF-B), a co-activator of Serum Response Factor (SRF). Phosphorylation of MRTF-B is required for its nuclear translocation after exercise and is accompanied by the transcription of the SRF target gene Fos. In addition, high-intensity exercise also remodels chromatin at specific SRF target gene loci through the phosphorylation of histone 3 on serine 10 in myonuclei of both mice and humans. Ablation of the MAP kinase member MSK1/2 is sufficient to prevent this histone phosphorylation, reduce induction of SRF-target genes, and prevent increases in protein synthesis after exercise.CONCLUSION: Our results identify a new exercise signaling fingerprint in vivo, instrumental for exercise-induced protein synthesis and potentially muscle growth.
- Subjects :
- 0301 basic medicine
Male
Serum Response Factor
protein synthesis
Physiology
030204 cardiovascular system & hematology
Muscle hypertrophy
histone phosphorylation
03 medical and health sciences
Mice
0302 clinical medicine
Physical Conditioning, Animal
Serum response factor
medicine
Regular Paper
Animals
Humans
skeletal muscle
Muscle, Skeletal
Transcription factor
biology
exercise
Chemistry
Regular Papers
Skeletal muscle
Exercise
SRF
Chromatin
Cell biology
Mice, Inbred C57BL
030104 developmental biology
Histone
Histone phosphorylation
medicine.anatomical_structure
Protein Biosynthesis
biology.protein
Phosphorylation
Histone Phosphorylation
Protein Synthesis
Skeletal Muscle
serum response factor
Signal Transduction
Transcription Factors
Subjects
Details
- ISSN :
- 17481716
- Volume :
- 230
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Acta physiologica (Oxford, England)
- Accession number :
- edsair.doi.dedup.....450452341b1861bbeb67acbab8e19ace