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The assay of dipeptides using fluorescamine and its application to determining dipeptidase activity
- Source :
- Analytical Biochemistry. 68:161-166
- Publication Year :
- 1975
- Publisher :
- Elsevier BV, 1975.
-
Abstract
- The differing effect of pH on the reaction of fluorescamine with amino acids and peptides has been utilized to develop a method for the quantitative estimation of peptides in the presence of amino acids. This method has been applied to the measurement of dipeptidase activities in human jejunal aspirates.
- Subjects :
- chemistry.chemical_classification
Dipeptidase
Dipeptidases
Chromatography
biology
Biophysics
Dipeptides
Cell Biology
Hydrogen-Ion Concentration
Dipeptidase activity
Fluorescamine
Biochemistry
Amino acid
Kinetics
chemistry.chemical_compound
Jejunum
Spectrometry, Fluorescence
chemistry
biology.protein
Humans
Molecular Biology
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....44ea50eac0bf051357a1184ef0d2e38c
- Full Text :
- https://doi.org/10.1016/0003-2697(75)90690-9