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miRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution
- Source :
- Cellular Physiology and Biochemistry, Vol 49, Iss 5, Pp 2088-2098 (2018)
- Publication Year :
- 2018
- Publisher :
- S. Karger AG, 2018.
-
Abstract
- Background/Aims: Based on the theory of constitution in Traditional Chinese Medicine (TCM), the Chinese Han population has been classified into nine constitutions. Of these, Yang deficiency constitution mainly exhibit cold intolerance while Yin deficiency constitution mainly exhibit heat intolerance. Some studies have been carried out to explore the modern genetic and biological basis of such constitution classification, but more remains to be done. MicroRNA (miRNA) serves as post-transcriptional regulators of gene expression and may play a role in the classification process. Here, we examined miRNA expression profile of saliva to further improve the comprehensiveness of constitution classification. Methods: Saliva was collected from Chinese Han individuals with Yang deficiency, Yin deficiency and Balanced constitutions (n=5 each), and miRNA expression profile was determined using the Human miRNA OneArray®v7. Based on 1.5 Fold change, means log2|Ratio|≥0.585 and P-value< 0.05, differentially expressed miRNA was screened. Target genes were predicted using DIANA-TarBasev7.0 and analysis of KEGG pathway was carried out using DIANA-mirPathv.3. Results: We found that 81 and 98 differentially expressed miRNAs were screened in Yang deficiency and Yin deficiency constitution, respectively. Among them, 16 miRNAs were identical and the others were unique. In addition, the target genes that are regulated by the unique miRNAs were significantly enriched in 27 and 20 signaling pathways in Yang deficiency and Yin deficiency constitution, respectively. Thyroid hormone signaling pathway is present in both constitutions. These unique miRNAs that regulated target genes of thyroid hormone signaling pathway may be associated with cold intolerance or heat intolerance. Conclusion: The results of our study show that Yang deficiency and Yin deficiency constitutions exhibit systematic differences in miRNA expression profile. Moreover, the distinct characteristics of TCM constitution may be explained, in part, by differentially expressed miRNAs.
- Subjects :
- Adult
Male
0301 basic medicine
Physiology
Traditional Chinese medicine
Biology
lcsh:Physiology
lcsh:Biochemistry
03 medical and health sciences
microRNA
Gene expression
medicine
Cluster Analysis
Humans
lcsh:QD415-436
Medicine, Chinese Traditional
KEGG
Saliva
Gene
Oligonucleotide Array Sequence Analysis
miRNA
Genetics
Principal Component Analysis
Heat intolerance
lcsh:QP1-981
humanities
Fold change
MicroRNAs
Yin Deficiency
Yang Deficiency
030104 developmental biology
Tcm constitution
Female
Target genes
medicine.symptom
Signal transduction
Transcriptome
Signal Transduction
Subjects
Details
- ISSN :
- 14219778 and 10158987
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Cellular Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....44d6d50e01a2467d31b32898b09671fb