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Micro-fractional epidermal powder delivery for improved skin vaccination
- Source :
- Journal of Controlled Release. 192:310-316
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Skin vaccination has gained increasing attention in the last two decades due to its improved potency compared to intramuscular vaccination. Yet, the technical difficulty and frequent local reactions hamper its broad application in the clinic. In the current study, micro-fractional epidermal powder delivery (EPD) is developed to facilitate skin vaccination and minimize local adverse effects. EPD is based on ablative fractional laser or microneedle treatment of the skin to generate microchannel (MC) arrays in the epidermis followed by topical application of powder drug/vaccine-coated array patches to deliver drug/vaccine into the skin. The novel EPD delivered more than 80% sulforhodamine b (SRB) and model antigen ovalbumin (OVA) into murine, swine, and human skin within 1 h. EPD of OVA induced anti-OVA antibody titer at a level comparable to intradermal (ID) injection and was much more efficient than tape stripping in both delivery efficiency and immune responses. Strikingly, the micro-fractional delivery significantly reduced local side effects of LPS/CpG adjuvant and BCG vaccine, leading to complete skin recovery. In contrast, ID injection induced severe local reactions that persisted for weeks. While reducing local reactogenicity, EPD of OVA/LPS/CpG and BCG vaccine generated a comparable humoral immune response to ID injection. EPD of vaccinia virus encoding OVA induced significantly higher and long-lasting interferon γ-secreting CD8 + T cells than ID injection. In conclusion, EPD represents a promising technology for needle-free, painless skin vaccination with reduced local reactogenicity and at least sustained immunogenicity.
- Subjects :
- Injections, Intradermal
Microinjections
Ovalbumin
Swine
CpG Oligodeoxynucleotide
Pharmaceutical Science
Human skin
Administration, Cutaneous
Article
Mice
Immune system
Adjuvants, Immunologic
Antigen
Animals
Humans
Medicine
Skin
Mice, Inbred BALB C
Vaccines
Reactogenicity
Rhodamines
business.industry
Lasers
Immunogenicity
Vaccination
Needles
Immunology
business
BCG vaccine
Subjects
Details
- ISSN :
- 01683659
- Volume :
- 192
- Database :
- OpenAIRE
- Journal :
- Journal of Controlled Release
- Accession number :
- edsair.doi.dedup.....44d29cf04c5932320ec5d8f58a037e44
- Full Text :
- https://doi.org/10.1016/j.jconrel.2014.08.006