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Echinacoside exerts anti-tumor activity via the miR-503-3p/TGF-β1/Smad aixs in liver cancer
- Source :
- Cancer Cell International, Cancer Cell International, Vol 21, Iss 1, Pp 1-9 (2021)
- Publication Year :
- 2020
-
Abstract
- Background Echinacoside (ECH) is the main active ingredient of Cistanches Herba, which is known to have therapeutic effects on metastatic tumors. However, the effects of ECH on liver cancer are still unclear. This study was to investigate the effects of ECH on the aggression of liver cancer cells. Methods Two types of liver cancer cells Huh7 and HepG2 were treated with different doses of ECH at different times and gradients. MTT and colony formation assays were used to determine the effects of ECH on the viability of Huh7 and HepG2 cells. Transwell assays and flow cytometry assays were used to detect the effects of ECH treatment on the invasion, migration, apoptosis and cell cycle of Huh7 and HepG2 cells. Western blot analysis was used to detect the effects of ECH on the expression levels of TGF-β1, smad3, smad7, apoptosis-related proteins (Caspase-3, Caspase-8), and Cyto C in liver cancer cells. The relationship between miR-503-3p and TGF-β1 was detected using bioinformatics analysis and Luciferase reporter assay. Results The results showed that ECH inhibited the proliferation, invasion and migration of Huh7 and HepG2 cells in a dose- and time-dependent manner. Moreover, we found that ECH caused Huh7 and HepG2 cell apoptosis by blocking cells in S phase. Furthermore, the expression of miR-503-3p was found to be reduced in liver tumor tissues, but ECH treatment increased the expression of miR-503-3p in Huh7 and HepG2 cells. In addition, we found that TGF-β1 was identified as a potential target of miR-503-3p. ECH promoted the activation of the TGF-β1/Smad signaling pathway and increased the expression levels of Bax/Bcl-2. Moreover, ECH could trigger the release of mitochondrial Cyto C, and cause the reaction Caspases grade. Conclusions This study demonstrates that ECH exerts anti-tumor activity via the miR-503-3p/TGF-β1/Smad aixs in liver cancer, and provides a safe and effective anti-tumor agent for liver cancer.
- Subjects :
- Cancer Research
Liver tumor
Apoptosis
SMAD
Flow cytometry
03 medical and health sciences
TGF-β1/smad
0302 clinical medicine
Western blot
Genetics
medicine
MiR-503-3p
RC254-282
Caspase
030304 developmental biology
0303 health sciences
QH573-671
biology
medicine.diagnostic_test
Chemistry
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Cell cycle
medicine.disease
Echinacoside
Oncology
030220 oncology & carcinogenesis
biology.protein
Cancer research
Cytology
Liver cancer
Primary Research
Subjects
Details
- ISSN :
- 14752867
- Volume :
- 21
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cancer cell international
- Accession number :
- edsair.doi.dedup.....44ca3e3e6d3faeda882295f3c458369c