Genetic fidelity of regenerated plants via shoot regeneration of muskmelon by inter simple sequence repeat and flow cytometry

A new and efficient method of direct shoot proliferation was developed here in two muskmelon genotypes (Cucumis melo L.), including ‘Khatooni’ and ‘Sooski Sabz’ (100%) using 4-day-old cotyledonary explants in vitro. The regeneration of muskmelon was evaluated on Murashige and Skoog (MS) medium supplemented separately with 17 treatments as combinations of two auxins (1-Naphthaleneacetic acid [NAA] and Naphthoxyacetic acid [NOA]) and four cytokinins (Benzylaminopurine [BAP], Thidiazuron [TDZ], Kinetin [KIN], and N-(2-Chloro-4-pyridyl)-N′-phenylurea [CPPU]). The treatments were experimented on 4- and 8-day-old cotyledon explants. A significant degree of organogenesis occurred in 4-day-old cotyledons (P