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Dependence of oxygen-tolerant nitrogenase activity on divalent cations in Azotobacter vinelandii

Authors :
J B Peterson
Source :
Journal of Bacteriology. 174:3399-3402
Publication Year :
1992
Publisher :
American Society for Microbiology, 1992.

Abstract

Nitrogenase activity of washed Azotobacter vinelandii cells was enhanced by the addition of Ca2+ and Mg2+, and the enhancement increased with the O2 concentration. In assays provided with a level of O2 that was initially supraoptimal and inhibitory to nitrogenase activity, the addition of Ca2+ or Mg2+ affected both the maximum respiration rate (Vmax) of the cells and the apparent affinity [KS(O2)] of cell respiration for O2. Changes in these parameters correlated with changes in nitrogenase activity. Aeration-dependent increases in Vmax and KS(O2) were inhibited by rifampin and chloramphenicol and were also observed in ammonium-grown cultures.

Details

ISSN :
10985530 and 00219193
Volume :
174
Database :
OpenAIRE
Journal :
Journal of Bacteriology
Accession number :
edsair.doi.dedup.....44ae07e323dd133303be4c7c1311761c
Full Text :
https://doi.org/10.1128/jb.174.10.3399-3402.1992