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Up-regulation of apoptotic- and cell survival-related gene pathways following exposures of western corn rootworm to B. thuringiensis crystalline pesticidal proteins in transgenic maize roots
- Source :
- BMC Genomics, BMC Genomics, BioMed Central, 2021, 22 (1), pp.639. ⟨10.1186/s12864-021-07932-4⟩, BMC Genomics, Vol 22, Iss 1, Pp 1-27 (2021)
- Publication Year :
- 2021
- Publisher :
- BioMed Central, 2021.
-
Abstract
- Background Resistance of pest insect species to insecticides, including B. thuringiensis (Bt) pesticidal proteins expressed by transgenic plants, is a threat to global food security. Despite the western corn rootworm, Diabrotica virgifera virgifera, being a major pest of maize and having populations showing increasing levels of resistance to hybrids expressing Bt pesticidal proteins, the cell mechanisms leading to mortality are not fully understood. Results Twenty unique RNA-seq libraries from the Bt susceptible D. v. virgifera inbred line Ped12, representing all growth stages and a range of different adult and larval exposures, were assembled into a reference transcriptome. Ten-day exposures of Ped12 larvae to transgenic Bt Cry3Bb1 and Gpp34/Tpp35Ab1 maize roots showed significant differential expression of 1055 and 1374 transcripts, respectively, compared to cohorts on non-Bt maize. Among these, 696 were differentially expressed in both Cry3Bb1 and Gpp34/Tpp35Ab1 maize exposures. Differentially-expressed transcripts encoded protein domains putatively involved in detoxification, metabolism, binding, and transport, were, in part, shared among transcripts that changed significantly following exposures to the entomopathogens Heterorhabditis bacteriophora and Metarhizium anisopliae. Differentially expressed transcripts in common between Bt and entomopathogen treatments encode proteins in general stress response pathways, including putative Bt binding receptors from the ATP binding cassette transporter superfamily. Putative caspases, pro- and anti-apoptotic factors, as well as endoplasmic reticulum (ER) stress-response factors were identified among transcripts uniquely up-regulated following exposure to either Bt protein. Conclusions Our study suggests that the up-regulation of genes involved in ER stress management and apoptotic progression may be important in determining cell fate following exposure of susceptible D. v. virgifera larvae to Bt maize roots. This study provides novel insights into insect response to Bt intoxication, and a possible framework for future investigations of resistance mechanisms.
- Subjects :
- 0106 biological sciences
Cry3Bb1
Pesticidal proteins
Diabrotica
Cell Survival
Bacillus thuringiensis
Apoptosis
Genetically modified crops
QH426-470
01 natural sciences
Zea mays
Transcriptome
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
Insecticide Resistance
03 medical and health sciences
Gene expression
Genetics
Animals
Pesticides
Pest Control, Biological
Gene
030304 developmental biology
2. Zero hunger
0303 health sciences
Genetically modified maize
biology
Research
Stress response
biology.organism_classification
Plants, Genetically Modified
Up-Regulation
Coleoptera
Endotoxins
010602 entomology
Western corn rootworm
Cell fate
Larva
TP248.13-248.65
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....449bdcec8d82bdfd69d81c7c52fff7be