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Chemokine Receptor 8 Can Distinguish Antineutrophil Cytoplasmic Antibody–Associated Vasculitis From Infectious Complications

Authors :
Yukako Akiyama
Mitsuhiro Sato
Toshinobu Sato
Satoru Sanada
Yoshio Taguma
Source :
Kidney International Reports
Publication Year :
2019
Publisher :
Elsevier BV, 2019.

Abstract

Introduction Diagnosing vasculitis is frequently difficult because its clinical symptoms are similar to those of common infectious diseases and other inflammatory disorders. This study focused on chemokine receptor 8 (CCR8) in peripheral blood mononuclear cells to find a new biomarker that distinguishes vasculitis from infectious complications. Methods A cross-sectional study was conducted among 113 patients with systemic vasculitis who were referred to Japan Health Care Organization Sendai Hospital from 2014 to 2016, including those with antineutrophil cytoplasmic antibody (ANCA)−associated vasculitis, anti−glomerular basement membrane disease, lupus nephritis, and Henoch-Schonlein purpura. Peripheral blood mononuclear cells were extracted from blood, and CCR8 expression was examined by real time polymerase chain reaction and flow cytometry. Results CCR8 gene expression was significantly higher in patients with ANCA-associated vasculitis, which was confirmed by upregulated CCR8 protein expression in flow cytometry (P < 0.001 and P = 0.01, respectively). Neither lupus nephritis nor Henoch-Schonlein purpura showed upregulated CCR8. Elevated CCR8 in the active phase decreased significantly in remission (P = 0.002), which was correlated with decreased serum inflammatory markers. Despite elevated serological inflammatory markers, the CCR8 levels at the time of infection, including bacterial, viral, and fungal, did not increase, indicating that infectious complications did not affect CCR8 expression (P = 0.02). Conclusion CCR8 in peripheral blood mononuclear cells may be a useful diagnostic marker for ANCA-associated vasculitis to differentiate between active vasculitis and infectious inflammation.

Details

ISSN :
24680249
Volume :
4
Database :
OpenAIRE
Journal :
Kidney International Reports
Accession number :
edsair.doi.dedup.....4492eae22bdf37d79dde518a6f6e0939
Full Text :
https://doi.org/10.1016/j.ekir.2018.11.003