Back to Search
Start Over
Molecular Cloning of a Second Human Stanniocalcin Homologue (STC2)
- Source :
- Biochemical and Biophysical Research Communications. 250:252-258
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- Stanniocalcin (STC) is a Ca- and phosphate-regulating hormone produced by the corpuscles of Stannius in bony fishes. The mammalian homologue of STC has recently been reported (STC1), which stimulates the phosphate uptake of kidney. Here we report the cloning of a second mammalian stanniocalcin (STC2) from the human osteosarcoma cDNA library. STC2 has 302 amino acid residues with 34% identity with STC1 and eel STC. STC2 has a conserved N-glycosylation site and is rich in cysteines as is the case with other stanniocalcins. STC2 has the same exon–intron boundaries as STC1. The culture medium of STC2-transfected CHO cells inhibited the promoter activity of Na-phosphate cotransporter (NaPi-3) and also inhibited the phosphate uptake of a kidney cell line (OK cells). Therefore, the function of STC2 seems to be opposite to that of STC1 on Na-phosphate cotransporter. Northern blot analysis revealed multiple transcripts in number of human tissues with high levels being present in skeletal muscle and heart. STC2 was also expressed in mice widely and its expression was lower in hypophosphatemic mice (Hypmice) in many organs. We have cloned a widely expressed new human stanniocalcin homologue which suppressed the expression of renal Na-phosphate cotransporter.
- Subjects :
- Molecular Sequence Data
Biophysics
Sequence Homology
Biology
Molecular cloning
Biochemistry
Mice
medicine
Animals
Humans
STC1
Amino Acid Sequence
Northern blot
Cloning, Molecular
Molecular Biology
Glycoproteins
Cloning
Kidney
Base Sequence
Genome, Human
cDNA library
Chinese hamster ovary cell
Intracellular Signaling Peptides and Proteins
Cell Biology
Molecular biology
Hormones
medicine.anatomical_structure
Intercellular Signaling Peptides and Proteins
Cotransporter
Sequence Analysis
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 250
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....4467fc2e6f909fac6ee2747f21bc2ffe