Back to Search
Start Over
Prevalence, Genetic Diversity, Antimicrobial Resistance, and Toxigenic Profile of Vibrio vulnificus Isolated from Aquatic Environments in Taiwan
- Source :
- Antibiotics, Antibiotics, Vol 10, Iss 505, p 505 (2021), Volume 10, Issue 5
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Vibrio vulnificus is a gram-negative, opportunistic human pathogen associated with life-threatening wound infections and is commonly found in warm coastal marine water environments, globally. In this study, two fishing harbors and three tributaries of the river basin were analyzed for the prevalence of V. vulnificus in the water bodies and shellfish that are under the pressure of external pollutions. The average detection rate of V. vulnificus in the river basins and fishing harbors was 8.3% and 4.2%, respectively, in all seasons. A total of nine strains of V. vulnificus were isolated in pure cultures from 160 samples belonging to river basins and fishing harbors to analyze the antibiotic susceptibility, virulence gene profiles, and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) fingerprinting. All isolates were susceptible to 10 tested antibiotics. The genotypic characterization revealed that 11.1% (n = 1/9) strain was nonvirulent, whereas 88.9% (n = 8/9) isolates were virulent strains, which possessed the four most prevalent toxin genes such as vcgC (88.9%), 16S B (88.9%), vvhA (88.9%), and manIIA (88.9%), followed by nanA (77.8%), CPS1 (66.7), and PRXII (44.4%). Additionally, ERIC-PCR fingerprinting grouped these nine isolates into two main clusters, among which the river basin isolates showed genetically diverse profiles, suggesting multiple sources of V. vulnificus. Ultimately, this study highlighted the virulent strains of V. vulnificus in the coastal aquatic environments of Taiwan, harboring a potential risk of infection to human health through water-borne transmission.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Veterinary medicine
030106 microbiology
Virulence
Human pathogen
RM1-950
Vibrio vulnificus
Biochemistry
Microbiology
Article
03 medical and health sciences
Intergenic region
Antibiotic resistance
Genotype
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Shellfish
Genetic diversity
biology
virulence gene
fungi
biology.organism_classification
bacterial infections and mycoses
030104 developmental biology
Infectious Diseases
Therapeutics. Pharmacology
ERIC-PCR
antibiotic susceptibility
aquatic environment
Subjects
Details
- Language :
- English
- ISSN :
- 20796382
- Volume :
- 10
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Antibiotics
- Accession number :
- edsair.doi.dedup.....44616418f9d9f128d5092c37ad8aa2f9