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Quantitative spatial analysis of haematopoiesis-regulating stromal cells in the bone marrow microenvironment by 3D microscopy
- Source :
- Nature Communications, Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018), Nature Communications, 9 (1)
- Publication Year :
- 2017
-
Abstract
- Sinusoidal endothelial cells and mesenchymal CXCL12-abundant reticular cells are principal bone marrow stromal components, which critically modulate haematopoiesis at various levels, including haematopoietic stem cell maintenance. These stromal subsets are thought to be scarce and function via highly specific interactions in anatomically confined niches. Yet, knowledge on their abundance, global distribution and spatial associations remains limited. Using three-dimensional quantitative microscopy we show that sinusoidal endothelial and mesenchymal reticular subsets are remarkably more abundant than estimated by conventional flow cytometry. Moreover, both cell types assemble in topologically complex networks, associate to extracellular matrix and pervade marrow tissues. Through spatial statistical methods we challenge previous models and demonstrate that even in the absence of major specific interaction forces, virtually all tissue-resident cells are invariably in physical contact with, or close proximity to, mesenchymal reticular and sinusoidal endothelial cells. We further show that basic structural features of these stromal components are preserved during ageing.<br />Nature Communications, 9 (1)<br />ISSN:2041-1723
- Subjects :
- 0301 basic medicine
Aging
Statistical methods
General Physics and Astronomy
Cell Count
Mice
Reticular cell
Bone Marrow
Cell Movement
Femur
Stem Cell Niche
lcsh:Science
Microscopy
Multidisciplinary
10042 Clinic for Diagnostic and Interventional Radiology
3100 General Physics and Astronomy
Cell biology
Extracellular Matrix
Haematopoiesis
medicine.anatomical_structure
Cellular Microenvironment
Stem cell
Confocal microscopy
Image processing
Stem-cell niche
Cell type
Stromal cell
Science
610 Medicine & health
1600 General Chemistry
Genetics and Molecular Biology
Bone Marrow Cells
Biology
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
Imaging, Three-Dimensional
1300 General Biochemistry, Genetics and Molecular Biology
medicine
Animals
Mesenchymal stem cell
Endothelial Cells
Mesenchymal Stem Cells
General Chemistry
Hematopoietic Stem Cells
Hematopoiesis
Mice, Inbred C57BL
030104 developmental biology
Reticular connective tissue
10032 Clinic for Oncology and Hematology
General Biochemistry
lcsh:Q
Bone marrow
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature communications
- Accession number :
- edsair.doi.dedup.....443d33f9a68f792ace625b6d704172b5