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Spatiotemporal transcriptome of the human brain
- Source :
- Nature
- Publication Year :
- 2011
-
Abstract
- Summary Here we report the generation and analysis of genome-wide exon-level transcriptome data from 16 brain regions comprising the cerebellar cortex, mediodorsal nucleus of the thalamus, striatum, amygdala, hippocampus, and 11 areas of the neocortex. The dataset was generated from 1,340 tissue samples collected from one or both hemispheres of 57 postmortem human brains, spanning from embryonic development to late adulthood and representing males and females of multiple ethnicities. We also performed genotyping of 2.5 million SNPs and assessed copy number variations for all donors. Approximately 86% of protein-coding genes were found to be expressed using stringent criteria, and over 90% of these were differentially regulated at the whole transcript or exon level across regions and/or time. The majority of these spatiotemporal differences occurred before birth, followed by an increase in the similarity among regional transcriptomes during postnatal lifespan. Genes were organized into functionally distinct co-expression networks, and sex differences were present in gene expression and exon usage. Finally, we demonstrate how these results can be used to profile trajectories of genes associated with neurodevelopmental processes, cell types, neurotransmitter systems, autism, and schizophrenia, as well as to discover associations between SNPs and spatiotemporal gene expression. This study provides a comprehensive, publicly available dataset on the spatiotemporal human brain transcriptome and new insights into the transcriptional foundations of human neurodevelopment.
- Subjects :
- Adult
Quality Control
Male
Aging
Time Factors
Adolescent
Quantitative Trait Loci
Gene regulatory network
Single-nucleotide polymorphism
Computational biology
Biology
Article
Transcriptome
03 medical and health sciences
Exon
Young Adult
0302 clinical medicine
Fetus
medicine
Humans
Gene Regulatory Networks
gene expression
brain development
microarray
human brain
Child
Gene
030304 developmental biology
Aged
Genetics
Regulation of gene expression
Aged, 80 and over
0303 health sciences
Sex Characteristics
Multidisciplinary
Gene Expression Profiling
human brain, transcriptome
Infant
Brain
Gene Expression Regulation, Developmental
Human brain
Exons
Middle Aged
Gene expression profiling
medicine.anatomical_structure
Child, Preschool
Female
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14764687 and 00280836
- Volume :
- 478
- Issue :
- 7370
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....443aa341ae3ec24545ae298da40b1bdd