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Limited influence of the microbiome on the transcriptional profile of female Aedes aegypti mosquitoes
- Source :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- The microbiome is an assemblage of microorganisms living in association with a multicellular host. Numerous studies have identified a role for the microbiome in host physiology, development, immunity, and behaviour. The generation of axenic (germ-free) and gnotobiotic model systems has been vital to dissecting the role of the microbiome in host biology. We have previously reported the generation of axenic Aedes aegypti mosquitoes, the primary vector of several human pathogenic viruses, including dengue virus and Zika virus. In order to better understand the influence of the microbiome on mosquitoes, we examined the transcriptomes of axenic and conventionally reared Ae. aegypti before and after a blood meal. Our results suggest that the microbiome has a much lower effect on the mosquito’s gene expression than previously thought with only 170 genes influenced by the axenic state, while in contrast, blood meal status influenced 809 genes. The pattern of expression influenced by the microbiome is consistent with transient changes similar to infection rather than sweeping physiological changes. While the microbiome does seem to affect some pathways such as immune function and metabolism, our data suggest the microbiome is primarily serving a nutritional role in development with only minor effects in the adult.
- Subjects :
- 0301 basic medicine
Transcription, Genetic
030231 tropical medicine
lcsh:Medicine
Mosquito Vectors
Aedes aegypti
Dengue virus
medicine.disease_cause
Article
Zika virus
qu_58.5
03 medical and health sciences
qu_58.7
0302 clinical medicine
Aedes
Immunity
medicine
qx_525
Animals
Germ-Free Life
Microbiome
lcsh:Science
Transcriptomics
Axenic
Genetics
qx_4
Multidisciplinary
qw_4
biology
Axenic Culture
Host (biology)
Microbiota
lcsh:R
biology.organism_classification
Blood meal
Diet
Blood
Drosophila melanogaster
Gene Ontology
030104 developmental biology
Larva
lcsh:Q
Female
Sugars
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
- Accession number :
- edsair.doi.dedup.....441eca62a363c8aae5e1dd10a6f3b75a