Back to Search
Start Over
A dps promoter based expression system for improved solubility of expressed proteins in Escherichia coli
- Source :
- Biotechnology and Bioprocess Engineering. 19:790-797
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- Escherichia coli is widely used for recombinant protein production due to its well established genetic manipulation techniques and cost effectiveness of the associated production processes. Soluble expression of heterologous recombinant proteins constitutes a major problem in the deployment of bacterial expression systems. We have developed a dps promoter based expression system in E. coli for improved solubility of expressed proteins. The resulting expression system was found to be superior to the IPTG inducible T7 promoter based pET expression system for production of soluble beta-galactosidase, tdTomato, and mCherry. The dps promoter based expression system was shown to be functional in most commonly used strains of E. coli without need for prior genetic manipulation of the host genome.
- Subjects :
- Protein Solubility
Cytoplasm
Cost effectiveness
Biomedical Engineering
lac operon
Heterologous
Bioengineering
Dps Promoter
Cloned Genes
Growth
Biology
medicine.disease_cause
Dna-Binding Protein
Applied Microbiology and Biotechnology
law.invention
law
medicine
Stationary-Phase
E.-Coli
Escherichia coli
Recombinant Protein Expression
Escherichia Coli
Improved solubility
Molecular biology
Heterologous Gene
Biochemistry
Metabolic Load
Rna-Polymerase
Recombinant DNA
Soluble Recombinant Proteins
Industrial and production engineering
mCherry
Biotechnology
Subjects
Details
- ISSN :
- 19763816 and 12268372
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Bioprocess Engineering
- Accession number :
- edsair.doi.dedup.....441ea916a78cda12e4f6b87d3e6a7dcb