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Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes
- Source :
- PLoS Neglected Tropical Diseases, Mucci, J, Carmona, S J, Volcovich, R, Altcheh, J, Bracamonte, E, Marco, J D, Nielsen, M, Buscaglia, C A & Aguero, F 2017, ' Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes ', P L o S Neglected Tropical Diseases (Online), vol. 11, no. 10, e0005972 . https://doi.org/10.1371/journal.pntd.0005972, CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET, PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0005972 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science, 2017.
-
Abstract
- Chagas Disease, caused by the protozoan Trypanosoma cruzi, is a major health and economic problem in Latin America for which no vaccine or appropriate drugs for large-scale public health interventions are yet available. Accurate diagnosis is essential for the early identification and follow up of vector-borne cases and to prevent transmission of the disease by way of blood transfusions and organ transplantation. Diagnosis is routinely performed using serological methods, some of which require the production of parasite lysates, parasite antigenic fractions or purified recombinant antigens. Although available serological tests give satisfactory results, the production of reliable reagents remains laborious and expensive. Short peptides spanning linear B-cell epitopes have proven ideal serodiagnostic reagents in a wide range of diseases. Recently, we have conducted a large-scale screening of T. cruzi linear B-cell epitopes using high-density peptide chips, leading to the identification of several hundred novel sequence signatures associated to chronic Chagas Disease. Here, we performed a serological assessment of 27 selected epitopes and of their use in a novel multipeptide-based diagnostic method. A combination of 7 of these peptides were finally evaluated in ELISA format against a panel of 199 sera samples (Chagas-positive and negative, including sera from Leishmaniasis-positive subjects). The multipeptide formulation displayed a high diagnostic performance, with a sensitivity of 96.3% and a specificity of 99.15%. Therefore, the use of synthetic peptides as diagnostic tools are an attractive alternative in Chagas’ disease diagnosis.<br />Author summary Chagas disease, caused by the parasite Trypanosoma cruzi, is a life-long and debilitating illness of major significance throughout Latin America, and an emergent threat to global public health. Diagnostic tests are key tools to support disease surveillance, and to ultimately help stop transmission of the parasite. However currently available diagnostic methods have several limitations. Identification of novel biomarkers with improved diagnostic characteristics is a main priority. Recently, we conducted a large-scale screening looking for new T. cruzi antigens using short peptides displayed on a solid support at high-density. This led to the identification of several hundred novel antigenic epitopes. In this work we validated the serodiagnostic performance of 27 of these against an extended panel of human serum samples. Based on this analysis, we developed a proof-of-principle multiplex diagnostic kit by combining different validated reactive peptides. Overall, our data support the applicability of high-density peptide microarrays for the rapid identification and mapping epitopes that could be readily translated into novel and useful tools for diagnosis of Chagas disease.
- Subjects :
- 0301 basic medicine
Proteomics
Microarrays
Physiology
Antibodies, Protozoan
Synthetic Biotechnology
Biochemistry
purl.org/becyt/ford/1 [https]
Epitopes
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Enzyme-Linked Immunoassays
Child
serodiagnosis
Protozoans
Immune System Proteins
lcsh:Public aspects of medicine
Eukaryota
Middle Aged
peptide
3. Good health
Infectious Diseases
Bioassays and Physiological Analysis
Engineering and Technology
Synthetic Biology
CIENCIAS NATURALES Y EXACTAS
Research Article
Neglected Tropical Diseases
Biotechnology
Chagas disease
Adult
Trypanosoma
lcsh:Arctic medicine. Tropical medicine
Adolescent
lcsh:RC955-962
Otras Ciencias Biológicas
Trypanosoma cruzi
030231 tropical medicine
Immunology
Antigens, Protozoan
Enzyme-Linked Immunosorbent Assay
Biology
Research and Analysis Methods
Ciencias Biológicas
03 medical and health sciences
Young Adult
antigen
SDG 3 - Good Health and Well-being
Diagnostic Medicine
medicine
Parasitic Diseases
Humans
Chagas Disease
Synthetic Peptides
Amino Acid Sequence
Antigens
purl.org/becyt/ford/1.6 [https]
Immunoassays
Protozoan Infections
Public Health, Environmental and Occupational Health
Organisms
Biology and Life Sciences
Proteins
lcsh:RA1-1270
medicine.disease
Tropical Diseases
Virology
Parasitic Protozoans
030104 developmental biology
Immunologic Techniques
Peptides
Epitope Mapping
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 11
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....44190dd6d9806247853aadf47329500a