Back to Search
Start Over
The Mobility of Phytochrome within Protonemal Tip Cells of the Moss Ceratodon purpureus, Monitored by Fluorescence Correlation Spectroscopy
- Source :
- Biophysical Journal
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Fluorescence correlation spectroscopy (FCS) is a versatile tool for investigating the mobilities of fluorescent molecules in cells. In this article, we show that it is possible to distinguish between freely diffusing and membrane-bound forms of biomolecules involved in signal transduction in living cells. Fluorescence correlation spectroscopy was used to measure the mobility of phytochrome, which plays a role in phototropism and polarotropism in protonemal tip cells of the moss Ceratodon purpureus. The phytochrome was loaded with phycoerythrobilin, which is fluorescent only in the phytochrome-bound state. Confocal laser scanning microscopy was used for imaging and selecting the xy measuring position in the apical zone of the tip cell. Fluorescence correlation was measured at ancient z-positions in the cell. Analysis of the diffusion coefficients by nonlinear least-square fits showed a subcellular fraction of phytochrome at the cell periphery with a sixfold higher diffusion coefficient than in the core fraction. This phytochrome is apparently bound to the membrane and probably controls the phototropic and polarotropic response.
- Subjects :
- Time Factors
Light
Green Fluorescent Proteins
Biophysics
Analytical chemistry
Phycoerythrobilin
Fluorescence correlation spectroscopy
Diffusion
Cell membrane
chemistry.chemical_compound
Phycobilins
medicine
Pyrroles
Phycobilin
Bile Pigments
Phototropism
Microscopy, Confocal
Ceratodon purpureus
Phytochrome
biology
Cell Membrane
Phycoerythrin
biology.organism_classification
Fluorescence
Bryopsida
Spectrometry, Fluorescence
medicine.anatomical_structure
Tetrapyrroles
chemistry
Cell Biophysics
Mutation
Signal Transduction
Subjects
Details
- ISSN :
- 00063495
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Biophysical Journal
- Accession number :
- edsair.doi.dedup.....44167a456eb7741e06a95e8679f0f5b5
- Full Text :
- https://doi.org/10.1529/biophysj.103.038521