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Preliminary X-ray analysis of twinned crystals of sarcosine dimethylglycine methyltransferase from Halorhodospira halochoris
- Source :
- Kallio, J P, Jänis, J, Nyyssölä, A, Hakulinen, N & Rouvinen, J 2009, ' Preliminary X-ray analysis of twinned crystals of sarcosine dimethylglycine methyltransferase from Halorhodospira halochoris ', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 65, no. 8, pp. 805-808 . https://doi.org/10.1107/S1744309109026232
- Publication Year :
- 2009
- Publisher :
- International Union of Crystallography, 2009.
-
Abstract
- Sarcosine dimethylglycine methyltransferase (EC 2.1.1.157) is an enzyme from the extremely halophilic anaerobic bacterium Halorhodospira halochoris. This enzyme catalyzes the twofold methylation of sarcosine to betaine, with S-­adenosylmethionine (AdoMet) as the methyl-group donor. This study presents the crystallization and preliminary X-ray analysis of recombinant sarcosine dimethylglycine methyltransferase produced in Escherichia coli. Mass spectroscopy was used to determine the purity and homogeneity of the enzyme material. Two different crystal forms, which initially appeared to be hexagonal and tetragonal, were obtained. However, on analyzing the diffraction data it was discovered that both crystal forms were pseudo-merohedrally twinned. The true crystal systems were monoclinic and orthorhombic. The monoclinic crystal diffracted to a maximum of 2.15 Å resolution and the orthorhombic crystal diffracted to 1.8 Å resolution.
- Subjects :
- Sarcosine
Twinning
Protein Conformation
Stereochemistry
Biophysics
Crystallography, X-Ray
Biochemistry
Mass Spectrometry
law.invention
Dimethylglycine
Crystal
chemistry.chemical_compound
Tetragonal crystal system
Betaine
Bacterial Proteins
Structural Biology
law
Genetics
Crystallization
Fourier Analysis
Chemistry
Methyltransferases
Condensed Matter Physics
Recombinant Proteins
Crystallography
Crystallization Communications
Halorhodospira halochoris
Sarcosine dimethylglycine methyltransferase
Orthorhombic crystal system
Ectothiorhodospiraceae
Monoclinic crystal system
Subjects
Details
- Language :
- English
- ISSN :
- 2053230X
- Volume :
- 65
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section F: Structural Biology Communications
- Accession number :
- edsair.doi.dedup.....43ee0f1c397daed76c6f5465efa696a3