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Effects of recombinant tumour necrosis factor on antibody-dependent eosinophil-mediated damage to Schistosoma japonicum larvae
- Source :
- Parasite Immunology. 14:605-616
- Publication Year :
- 1992
- Publisher :
- Wiley, 1992.
-
Abstract
- Human recombinant tumour necrosis factor (rTNF) enhanced monoclonal IgE-dependent eosinophil-mediated cytotoxicity to schistosomula of Schistosoma japonicum in a dose-dependent manner. The enhancing effect of rTNF was also observed for the antibody-dependent cytotoxicity of a human eosinophilic leukemia cell line, EoL-3, but not of another cell line, EoL-1. Observation by a slow-motion movie camera demonstrated that activated EoL-3 cells adhered to the surface of schistosomula by 6 h after incubation, which triggered intracellular movement of eosinophil granules. The granules were concentrated toward the surface of the larvae and then degranulation started. The cell membrane was left as a balloon-like remnant. Cell sorting analysis by FACStar indicated that the expression of receptors for C3bi (CR3) and low affinity FcR for IgE (Fc epsilon RII) increased on the surface of EoL-3 cells after stimulation with rTNF, while this was not observed for EoL-1 cells.
- Subjects :
- Immunology
Schistosoma japonicum
Rats, Sprague-Dawley
Mice
parasitic diseases
Tumor Cells, Cultured
medicine
Animals
Humans
Cytotoxicity
Antibody-dependent cell-mediated cytotoxicity
biology
Tumor Necrosis Factor-alpha
Antibody-Dependent Cell Cytotoxicity
Degranulation
Antibodies, Monoclonal
Immunoglobulin E
Cell sorting
Eosinophil
Flow Cytometry
biology.organism_classification
Recombinant Proteins
humanities
Rats
Eosinophils
medicine.anatomical_structure
Cell culture
Larva
biology.protein
Parasitology
Antibody
Subjects
Details
- ISSN :
- 13653024 and 01419838
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Parasite Immunology
- Accession number :
- edsair.doi.dedup.....43daadc72a3833d3bbfa0ecf5a24409e