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PFKFB4 controls embryonic patterning via Akt signalling independently of glycolysis
- Source :
- Nature Communications, Nature Communications, Nature Publishing Group, 2014, 6, pp.5953. ⟨10.1038/ncomms6953⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- International audience; How metabolism regulators play roles during early development remains elusive. Here we show that PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), a glycolysis regulator, is critical for controlling dorsal ectoderm global patterning in gastrulating frog embryos via a non-glycolytic function. PFKFB4 is required for dorsal ectoderm progenitors to proceed towards more specified fates including neural and non-neural ectoderm, neural crest or placodes. This function is mediated by Akt signalling, a major pathway that integrates cell homeostasis and survival parameters. Restoring Akt signalling rescues the loss of PFKFB4 in vivo. In contrast, glycolysis is not essential for frog development at this stage. Our study reveals the existence of a PFKFB4-Akt checkpoint that links cell homeostasis to the ability of progenitor cells to undergo differentiation, and uncovers glycolysis-independent functions of PFKFB4.
- Subjects :
- chemistry.chemical_classification
Cell signaling
Embryo, Nonmammalian
Multidisciplinary
Akt signalling
Phosphofructokinase-2
[SDV]Life Sciences [q-bio]
General Physics and Astronomy
Ectoderm
General Chemistry
PFKFB4
Biology
Embryonic Induction
Embryonic stem cell
General Biochemistry, Genetics and Molecular Biology
Cell biology
Oncogene Protein v-akt
medicine.anatomical_structure
Enzyme
Biochemistry
chemistry
medicine
Animals
Glycolysis
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications, Nature Publishing Group, 2014, 6, pp.5953. ⟨10.1038/ncomms6953⟩
- Accession number :
- edsair.doi.dedup.....43aa35de127028dd38b28ecd645d286b