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Varying degrees of phosphorylation determine microheterogeneity of the heavy neurofilament polypeptide (Nf-H)
- Source :
- Journal of Neuroimmunology. 14:135-148
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- Two-dimensional immunoblots revealed a spectrum of 200 kDa neurofilament polypeptides (Nf-H) of apparent molecular weights ranging from 200 to 170 kDa. The entire spectrum was stained immunocytochemically by three monoclonal antibodies specific for nonphosphorylated neurofilaments, while more restricted staining was revealed by four monoclonal antibodies specific for phosphorylated neurofilament epitopes. Treatment with increasing amounts of phosphatase suggested the existence of various forms of partially phosphorylated neurofilaments that posses phosphoepitopes that differ in their ease of dephosphorylation. Immunoprecipitation in low detergent concentration confirmed the existence of microheterogeneous forms of Nf-H that differed in extent of phosphorylation or in distribution of phosphorylated sites.
- Subjects :
- Paper
Neurofilament
Immunoprecipitation
medicine.drug_class
Immunology
Phosphatase
Monoclonal antibody
Antibodies
Epitope
Dephosphorylation
Epitopes
Intermediate Filament Proteins
Neurofilament Proteins
medicine
Immunology and Allergy
Phosphorylation
Staining and Labeling
Molecular mass
Chemistry
Antibodies, Monoclonal
Collodion
Molecular biology
Neurology
Biochemistry
Electrophoresis, Polyacrylamide Gel
Neurology (clinical)
Protein Processing, Post-Translational
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroimmunology
- Accession number :
- edsair.doi.dedup.....43972ba911143d85899e3b6acccb1ad0