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NKG2D ligands in inflammatory joint diseases: analysis in human samples and mouse models
- Source :
- Scopus-Elsevier, Europe PubMed Central
- Publication Year :
- 2020
- Publisher :
- Research Square Platform LLC, 2020.
-
Abstract
- Background NKG2D ligands (NKG2DLs) are stress-inducible molecules involved in multiple inflammatory settings. In this work, we quantified MICA, an NKG2DL, in the synovial fluid of patients suffering various arthritides and measured Nkg2dLs gene expression in murine models of acute joint inflammation. Methods Soluble MICA (sMICA) was quantified by ELISA is synovial fluids harvested from patients suffering osteoarthritis, rheumatoid arthritis, psoriatic arthritis, calcium pyrophosphate crystal arthritis, urate crystal arthritis and reactive arthritis. Transcripts encoding murine NKG2DLs were quantified by RT-qPCR in the joints of mouse models of rheumatoid arthritis, urate crystal arthritis and osteoarthritis. Results Marked overproduction of sMICA was observed in the synovial fluid of RA patients. Mouse studies highlighted the complex transcriptional regulation of Nkg2d ligands encoding genes depending on the inflammatory setting and microenvironment Conclusions sMICA quantification could be an interesting biomarker to identify acute inflammation in RA patients in which classical markers (ie, anti-citrullinated protein antibodies, ACPA) are undetectable.
- Subjects :
- Immunology
Arthritis
Inflammation
Osteoarthritis
NKG2D ligands
Ligands
Anti-Citrullinated Protein Antibodies
Arthritis, Rheumatoid
Mice
Psoriatic arthritis
Rheumatology
Rheumatoid
Synovial Fluid
medicine
Immunology and Allergy
Animals
Humans
Synovial fluid
MICA
joint inflammation
NK Cell Lectin-Like Receptor Subfamily K
Reactive arthritis
business.industry
medicine.disease
Rheumatoid arthritis
Biomarker (medicine)
medicine.symptom
business
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier, Europe PubMed Central
- Accession number :
- edsair.doi.dedup.....439455ad0c5e58919b25cb3ecb01f751