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A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells
- Source :
- Nucleic Acids Research
- Publication Year :
- 2008
- Publisher :
- Oxford University Press, 2008.
-
Abstract
- Phosphoglycerate kinase 2 (PGK2) is a germ cell-specific protein whose mRNA is translationally regulated in the mammalian testis. Using RNA affinity chromatography with the 3'-untranslated region (UTR) of Pgk2 mRNA and adult testis extracts, several associated proteins including a novel isoform of the AU-rich element RNA-binding protein and KH-type splicing regulatory protein (KSRP) were identified. KSRP, a protein of approximately 75 kDa, is widely expressed in somatic and germ cells where it is primarily nuclear. In addition to the approximately 75-kDa KSRP, a approximately 52-kD KSRP, t-KSRP, is present in the cytoplasm of a subpopulation of germ cells. t-KSRP binds directly to a 93-nt sequence (designated the F1 region) of the 3'-UTR of the Pgk2 mRNA and destabilizes Pgk2 mRNA constructs in testis extracts and in transfected cells. We conclude that this testicular variant of the multifunctional nucleic acid-binding protein, KSRP, serves as a decay-promoting factor for Pgk2 mRNA in male germ cells.
- Subjects :
- Gene isoform
Untranslated region
Male
Cytoplasm
Somatic cell
RNA Stability
RNA-binding protein
Nerve Tissue Proteins
Biology
Mice
Testis
Genetics
Animals
Humans
Protein Isoforms
Polypyrimidine tract-binding protein
RNA, Messenger
Molecular Biology
3' Untranslated Regions
Messenger RNA
Binding Sites
Three prime untranslated region
Genetic Variation
RNA-Binding Proteins
Molecular biology
Isoenzymes
Meiosis
Phosphoglycerate Kinase
RNA splicing
biology.protein
Trans-Activators
HeLa Cells
Polypyrimidine Tract-Binding Protein
Subjects
Details
- Language :
- English
- ISSN :
- 13624962 and 03051048
- Volume :
- 36
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....4380080487db14db065afff9a85fa8c1