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Human colon carcinoma, fibrosarcoma and leukemia cell lines produce tumor-associated trypsinogen
- Source :
- International Journal of Cancer. 47:592-596
- Publication Year :
- 1991
- Publisher :
- Wiley, 1991.
-
Abstract
- Previous studies have indicated that cyst fluid of ovarian tumors contains 2 trypsinogen isoenzymes, called tumor-associated trypsinogen-I (TAT-I) and trypsinogen-2 (TAT-2), the levels of which correlate with the degree of malignancy of the tumors. In addition, these cyst fluids contain large amounts of tumor-associated trypsin inhibitor (TATI), which is also expressed in many other human tumors. In the present study we examined the production of TAT-I, TAT-2 and TATI in 9 established tumor-cell lines. TAT-2 was produced by 5 cell lines. Its concentration in the conditioned medium of COLO 205 colon adenocarcinoma cells, K-562 erythroleukemia cells and fibrosarcoma cell lines HT 1080, 8387 and A 9733 was 460 micrograms/l, 9.8 micrograms/l, 21 micrograms/l, 8.8 micrograms/l and 0.24 micrograms/l, respectively. TAT-I was detectable in the conditioned medium of COLO 205 and HT 1080 cells at concentrations of 64 micrograms/l and 0.5 micrograms/l, respectively. TATI was detected only in the media of COLO 205 cells at a concentration of 23 micrograms/l. TAT-2 zymogen was purified from the conditioned medium of COLO 205 and HT 1080 cells by immunoaffinity chromatography. According to its aminoterminal amino acid sequence, a molecular mass of 28 kDa by SDS-PAGE, elution pattern in ion-exchange chromatography and ability to be activated by enteropeptidase, the zymogen is identical to that previously isolated from cyst fluid of ovarian tumors. In addition, we found that TAT-2 secretion could be down-regulated by dexamethasone in HT 1080 cells but not in COLO 205 cells. The abundant production of TAT-2 isoenzyme in different cancer cell lines suggests that it could contribute to the increased proteolytic activity of many human tumors.
- Subjects :
- Enteropeptidase
Cancer Research
medicine.medical_specialty
Trypsinogen
Fibrosarcoma
Trypsin inhibitor
Biology
Dexamethasone
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Zymogen
Tumor Cells, Cultured
medicine
Humans
030304 developmental biology
0303 health sciences
Leukemia
Molecular mass
Carcinoma
medicine.disease
Molecular biology
digestive system diseases
Extracellular Matrix
3. Good health
Isoenzymes
Endocrinology
Oncology
chemistry
Cell culture
030220 oncology & carcinogenesis
Colonic Neoplasms
Subjects
Details
- ISSN :
- 10970215 and 00207136
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- International Journal of Cancer
- Accession number :
- edsair.doi.dedup.....436ed5936bedfc3dc31f57e867e65271