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IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
- Publication Year :
- 2019
-
Abstract
- Tenascin-C (TNC), a very large multimeric glycoprotein, is overexpressed in human glioblastomas, leading to a highly motile and invasive phenotype of glioma cells. However, the regulation of TNC expression in glioma has remained unclear until now. Our data suggest that interleukin-33 (IL-33) may promote the accumulation of TNC protein by autocrine or paracrine modes of action in glioma. In the present study, the expression levels of TNC, IL-33, and ST2 were measured in glioma tissue specimens, and the impact of altered IL-33 expression on TNC was investigated in vitro and in vivo. In contrast with control treatment, IL-33 treatment increased TNC expression, and knockdown of IL-33 attenuated TNC expression in glioma cells. Furthermore, IL-33 induced the activation of nuclear factor κB (NF-κB) and increased the expression of TNC in U251 cells. In addition, blockage of the IL-33-ST2-NFκB pathway resulted in downregulation of TNC production. IL-33 promoted glioma cell invasion by stimulating the secretion of TNC. Similarly, knockdown of TNC inhibited the invasiveness of glioma cells. These findings provide a novel perspective on the role of the IL-33/NF-κB/TNC signalling pathway in supporting cancer progression. Thus, targeting the IL-33/NF-κB/TNC signalling pathway may be a useful therapeutic approach in glioma.
- Subjects :
- lcsh:Medicine
Gene Expression
Models, Biological
Article
03 medical and health sciences
Paracrine signalling
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Glioma
Cell Line, Tumor
medicine
Humans
Neoplasm Invasiveness
lcsh:Science
Autocrine signalling
030304 developmental biology
0303 health sciences
Gene knockdown
Multidisciplinary
biology
Chemistry
lcsh:R
Tenascin C
NF-kappa B
Tenascin
medicine.disease
musculoskeletal system
Interleukin-33
Immunohistochemistry
Interleukin-1 Receptor-Like 1 Protein
Hedgehog signaling pathway
CNS cancer
030220 oncology & carcinogenesis
biology.protein
Cancer research
lcsh:Q
Signal transduction
Glioblastoma
Biomarkers
Signal Transduction
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....436a751713a7225612ffbebb71d4dfca