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A fully automated robotic system for microinjection of zebrafish embryos
- Source :
- PLoS ONE, Vol 2, Iss 9, p e862 (2007), PLoS ONE
- Publication Year :
- 2007
- Publisher :
- Public Library of Science (PLoS), 2007.
-
Abstract
- As an important embodiment of biomanipulation, injection of foreign materials (e.g., DNA, RNAi, sperm, protein, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This paper presents a microrobotic system for fully automated zebrafish embryo injection, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility. Based on computer vision and motion control, the microrobotic system performs injection at a speed of 15 zebrafish embryos (chorion unremoved) per minute, with a survival rate of 98% (n = 350 embryos), a success rate of 99% (n = 350 embryos), and a phenotypic rate of 98.5% (n = 210 embryos). The sample immobilization technique and microrobotic control method are applicable to other biological injection applications such as the injection of mouse oocytes/embryos and Drosophila embryos to enable high-throughput biological and pharmaceutical research.
- Subjects :
- Computer Science/Systems and Control Theory
Embryo, Nonmammalian
animal structures
Microinjections
Science
02 engineering and technology
Computer Science/Applications
Bioinformatics
Animals, Genetically Modified
03 medical and health sciences
Automation
Animals
Zebrafish
Microinjection
030304 developmental biology
DNA Primers
0303 health sciences
Multidisciplinary
biology
Base Sequence
Embryo
Robotics
021001 nanoscience & nanotechnology
biology.organism_classification
Sperm
3. Good health
Cell biology
Robotic systems
Fully automated
embryonic structures
Zebrafish embryo
Medicine
Biotechnology/Bioengineering
0210 nano-technology
Control methods
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 2
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....436600f80b3868225f3c592b62876c1c