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A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing
- Source :
- Stem Cell Research, Vol 17, Iss 1, Pp 93-96 (2016)
- Publisher :
- The Author(s). Published by Elsevier B.V.
-
Abstract
- We have generated a MIXL1-eGFP reporter human embryonic stem cell (hESC) line using TALEN-based genome engineering. This line accurately traces endogenous MIXL1 expression via an eGFP reporter to mesendodermal precursor cells. The utility of the MIXL1-eGFP reporter hESC line lies in the prospective isolation, lineage tracing, and developmental and mechanistic studies of MIXL1+ cell populations.
- Subjects :
- Medicine(all)
0301 basic medicine
Transcription activator-like effector nuclease
Cell
Cell Biology
General Medicine
Biology
Embryonic stem cell
Molecular biology
Genome engineering
Green fluorescent protein
Cell biology
03 medical and health sciences
030104 developmental biology
medicine.anatomical_structure
Genome editing
lcsh:Biology (General)
Precursor cell
embryonic structures
medicine
MIXL1
lcsh:QH301-705.5
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 18735061
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....436063cbaf7bb26e24c9a9c69fdb36e6
- Full Text :
- https://doi.org/10.1016/j.scr.2016.05.010