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Postmortem mitochondrial membrane permeability transition assessment of apoptotic cell death in brain and liver of insulin resistant, ovariectomised rats
- Source :
- IBRO Neuroscience Reports, Vol 11, Iss, Pp 156-163 (2021), IBRO Neuroscience Reports
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- The adverse alterations in mitochondrial functions can affect neuronal function negatively, as they play a crucial role in neuronal plasticity and death. Direct measurements of mitochondrial activity, including membrane potential and ATP production, are not easily achieved in post-mortem brain and liver samples because most organ functions cease to work after death; in fact, with increasing post-mortem intervals (PMI), the brain and liver tissues deteriorate rapidly. Standard procedures of mitochondria isolation, protein determination expressed as BSA equivalent, and spectrophotometric assessment of pore opening at 540 nm were employed. Our results showed that (a) intact mitochondria may be isolated from rat brain and liver of these rats after storage in animal body (in situ) at −20 °C for 7 days (168 h, post-mortem), (b) some population of these mitochondria can still take up exogenous Ca2+ and (c) they can still resist osmotically induced large amplitude swelling in a suitable buffer. The need for mitochondrial purity, structural integrity and abundance for functional studies are common hindrances that can encumber mitochondrial research. Therefore, this study is significant to have shown that PMI up to 7 days did not extensively, diminish MMPT pore status in normal and diabetic, ovariectomised rats. This can be relevant for forensic data mining.
- Subjects :
- Membrane potential
education.field_of_study
Chemistry
General Neuroscience
Ovariectomy
Population
Diabetes
Insulin resistant
Neurosciences. Biological psychiatry. Neuropsychiatry
Mitochondrion
Mitochondrial membrane permeability transition
Cell biology
Apoptotic cell death
Neuroplasticity
education
Function (biology)
Articles from the Special Issue on Neuroscience in Africa
Edited by James O. Olopade
Mitochondrial membrane permeability transition (MMPT)
Post-mortem intervals
RC321-571
Subjects
Details
- Language :
- English
- ISSN :
- 26672421
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- IBRO Neuroscience Reports
- Accession number :
- edsair.doi.dedup.....436015cb767717df2d4f1e680562aaa7