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Escherichia coli growth under modeled reduced gravity
- Source :
- Microgravity - Science and Technology. 15:39-44
- Publication Year :
- 2004
- Publisher :
- Springer Science and Business Media LLC, 2004.
-
Abstract
- Bacteria exhibit varying responses to modeled reduced gravity that can be simulated by clino-rotation. When Escherichia coli was subjected to different rotation speeds during clino-rotation, significant differences between modeled reduced gravity and normal gravity controls were observed only at higher speeds (30-50 rpm). There was no apparent affect of removing samples on the results obtained. When E. coli was grown in minimal medium (at 40 rpm), cell size was not affected by modeled reduced gravity and there were few differences in cell numbers. However, in higher nutrient conditions (i.e., dilute nutrient broth), total cell numbers were higher and cells were smaller under reduced gravity compared to normal gravity controls. Overall, the responses to modeled reduced gravity varied with nutrient conditions; larger surface to volume ratios may help compensate for the zone of nutrient depletion around the cells under modeled reduced gravity.
- Subjects :
- Gravity (chemistry)
Indoles
Reduced Gravity
Rotation
Colony Count, Microbial
General Physics and Astronomy
Soil science
medicine.disease_cause
Nutrient
Escherichia coli
medicine
Nutrient broth
In Situ Hybridization, Fluorescence
Weightlessness Simulation
Fluorescent Dyes
Gravity, Altered
Staining and Labeling
Chemistry
Applied Mathematics
General Engineering
Culture Media
Volume (thermodynamics)
Modeling and Simulation
Subjects
Details
- ISSN :
- 18750494 and 09380108
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Microgravity - Science and Technology
- Accession number :
- edsair.doi.dedup.....4353977568272269caa2d94db3d29917