Back to Search
Start Over
Piracetam induces plasma membrane depolarization in rat brain synaptosomes
- Source :
- Neuroscience Letters. 553:206-210
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Piracetam is a cyclic derivative of γ-aminobutyric acid (GABA). It was the first nootropic drug approved for clinical use. However, mechanism of its action is still not clear. In present paper, I investigated effects of piracetam on neurotransmitter release, plasma membrane potential monitored by fluorescent dye DiSC3(5) and chloride transport monitored by fluorescent dye SPQ in rat brain synaptosomes. It was shown that piracetam (1 mM) induces slow weak plasma membrane depolarization. This effect was decreased on 43% and 58% by both AMPA/kainate receptor blockers NBQX (10 μM) and CNQX (100 μM), respectively, on 84% by GABA ionotropic receptor blocker picrotoxin (50 μM) and on 91% upon withdrawal of HCO(3-) ions from incubation medium. GABA (1 mM) and kainate (100 μM) were found not to produce changes of plasma membrane potential. Also, it was found that piracetam induces chloride efflux which seems to be the reason of depolarization. Thereby, piracetam induces depolarization of plasma membrane of isolated neuronal presynaptic endings by picrotoxin-sensitive way.
- Subjects :
- Male
Kainate receptor
AMPA receptor
Membrane Potentials
GABA Antagonists
chemistry.chemical_compound
Chlorides
Receptors, Kainic Acid
medicine
Animals
Benzothiazoles
Receptors, AMPA
Rats, Wistar
Nootropic Agents
Fluorescent Dyes
Membrane potential
Quinolinium Compounds
General Neuroscience
Cell Membrane
Brain
Cell Polarity
Piracetam
Biological Transport
Depolarization
Carbocyanines
chemistry
Biochemistry
CNQX
Biophysics
NBQX
Synaptosomes
Picrotoxin
medicine.drug
Subjects
Details
- ISSN :
- 03043940
- Volume :
- 553
- Database :
- OpenAIRE
- Journal :
- Neuroscience Letters
- Accession number :
- edsair.doi.dedup.....43513b6b5a6ac20ed7942a52e2c03b75