Back to Search
Start Over
Platelet-derived growth factors and fibroblast growth factors are mitogens for rat Schwann cells
- Source :
- The Journal of Cell Biology
- Publication Year :
- 1990
- Publisher :
- Rockefeller University Press, 1990.
-
Abstract
- Rat sciatic nerve Schwann cells in culture respond to a limited range of mitogens, including glial growth factor, transforming growth factors beta-1 and beta-2 (TGF-beta 1, TGF-beta 2), some cell membrane-associated factors, and to agents such as cholera toxin and forskolin which raise intracellular levels of cAMP. These responses require the presence of FCS, which exhibits little or no mitogenic activity in the absence of other factors. However, we recently found that forskolin greatly potentiates the mitogenic signal from TGFs-beta 1 and beta 2, raising the possibility that cAMP might couple other factors to mitogenesis. We have therefore screened a range of candidate mitogens using DNA synthesis assays. Other than TGFs-beta and glial growth factor, none of the factors tested were mitogenic in the presence of 10% serum alone. With the addition of forskolin, however, porcine PDGF, human PDGF, acidic and basic FGF were potent mitogens for rat Schwann cells, stimulating DNA synthesis and increasing cell number. Cholera toxin and dibutyrylcyclicAMP, but not 1,9-dideoxyforskolin, can substitute for forskolin indicating that the mitogenic effect is mediated via adenylyl cyclase activation. Porcine PDGF gave half-maximal stimulation at 15 pM, and human PGDF an equivalent response at 1 nM. Basic FGF was half maximal at 5 pM, acidic FGF at 1 nM. The recognition of PDGFs and FGFs as mitogens for Schwann cells has many implications for the study of Schwann cell proliferation in the development and regeneration of nerves, and in Schwann cell tumorigenesis.
- Subjects :
- DNA Replication
medicine.medical_specialty
Platelet-derived growth factor
medicine.medical_treatment
Schwann cell
Biology
Fibroblast growth factor
Schwann cell proliferation
chemistry.chemical_compound
Internal medicine
Cyclic AMP
medicine
Animals
Cells, Cultured
Platelet-Derived Growth Factor
Forskolin
Growth factor
Colforsin
Rats, Inbred Strains
Articles
Cell Biology
Sciatic Nerve
Recombinant Proteins
Culture Media
Rats
Cell biology
Fibroblast Growth Factors
Endocrinology
medicine.anatomical_structure
chemistry
biology.protein
Schwann Cells
Mitogens
Cell Division
Platelet-derived growth factor receptor
Transforming growth factor
Subjects
Details
- ISSN :
- 15408140 and 00219525
- Volume :
- 110
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....434e8a549723519a478c34d38457b092